4psl: Difference between revisions
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==Crystal structure of pfuThermo-DBP-RP1 (crystal form I)== | ==Crystal structure of pfuThermo-DBP-RP1 (crystal form I)== | ||
<StructureSection load='4psl' size='340' side='right' caption='[[4psl]], [[Resolution|resolution]] 3.50Å' scene=''> | <StructureSection load='4psl' size='340' side='right'caption='[[4psl]], [[Resolution|resolution]] 3.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4psl]] is a 4 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4psl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PSL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PSL FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4psl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4psl OCA], [https://pdbe.org/4psl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4psl RCSB], [https://www.ebi.ac.uk/pdbsum/4psl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4psl ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q8U208_PYRFU Q8U208_PYRFU] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Pyrococcus furiosus DSM 3638]] | ||
[[Category: | [[Category: Eppers D]] | ||
[[Category: | [[Category: Gahlei H]] | ||
[[Category: | [[Category: Loll B]] | ||
[[Category: Sohmen | [[Category: Sohmen D]] | ||
[[Category: Wahl | [[Category: Wahl MC]] | ||
[[Category: Wilson | [[Category: Wilson DN]] | ||
[[Category: | [[Category: Von Moeller H]] | ||
Revision as of 10:28, 8 February 2023
Crystal structure of pfuThermo-DBP-RP1 (crystal form I)Crystal structure of pfuThermo-DBP-RP1 (crystal form I)
Structural highlights
FunctionPublication Abstract from PubMedInstead of a classical single-stranded deoxyribonuleic acid (DNA)-binding protein (SSB), some hyperthermophilic crenarchaea harbor a non-canonical SSB termed ThermoDBP. Two related but poorly characterized groups of proteins, which share the ThermoDBP N-terminal DNA-binding domain, have a broader phylogenetic distribution and co-exist with ThermoDBPs and/or other SSBs. We have investigated the nucleic acid binding properties and crystal structures of representatives of these groups of ThermoDBP-related proteins (ThermoDBP-RPs) 1 and 2. ThermoDBP-RP 1 and 2 oligomerize by different mechanisms and only ThermoDBP-RP2 exhibits strong single-stranded DNA affinity in vitro. A crystal structure of ThermoDBP-RP2 in complex with DNA reveals how the NTD common to ThermoDBPs and ThermoDBP-RPs can contact the nucleic acid in a manner that allows a symmetric homotetrameric protein complex to bind single-stranded DNA molecules asymmetrically. While single-stranded DNA wraps around the surface or binds along channels of previously investigated SSBs, it traverses an internal, intersubunit tunnel system of a ThermoDBP-RP2 tetramer. Our results indicate that some archaea have acquired special SSBs for genome maintenance in particularly challenging environments. Entrapment of DNA in an intersubunit tunnel system of a single-stranded DNA-binding protein.,Ghalei H, Moeller HV, Eppers D, Sohmen D, Wilson DN, Loll B, Wahl MC Nucleic Acids Res. 2014 Apr 17. PMID:24744237[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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