4pof: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==PfMCM N-terminal domain without DNA== | ==PfMCM N-terminal domain without DNA== | ||
<StructureSection load='4pof' size='340' side='right' caption='[[4pof]], [[Resolution|resolution]] 2.65Å' scene=''> | <StructureSection load='4pof' size='340' side='right'caption='[[4pof]], [[Resolution|resolution]] 2.65Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4pof]] is a 6 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4pof]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4POF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4POF FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pof FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pof OCA], [https://pdbe.org/4pof PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pof RCSB], [https://www.ebi.ac.uk/pdbsum/4pof PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pof ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q8U3I4_PYRFU Q8U3I4_PYRFU] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 22: | Line 22: | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Pyrococcus furiosus DSM 3638]] | ||
[[Category: | [[Category: Bell SP]] | ||
[[Category: | [[Category: Enemark EJ]] | ||
[[Category: | [[Category: Epling LB]] | ||
[[Category: | [[Category: Froelich CA]] | ||
[[Category: | [[Category: Kang S]] | ||
Revision as of 10:21, 8 February 2023
PfMCM N-terminal domain without DNAPfMCM N-terminal domain without DNA
Structural highlights
FunctionPublication Abstract from PubMedThe ring-shaped MCM helicase is essential to all phases of DNA replication. The complex loads at replication origins as an inactive double-hexamer encircling duplex DNA. Helicase activation converts this species to two active single hexamers that encircle single-stranded DNA (ssDNA). The molecular details of MCM DNA interactions during these events are unknown. We determined the crystal structure of the Pyrococcus furiosus MCM N-terminal domain hexamer bound to ssDNA and define a conserved MCM-ssDNA binding motif (MSSB). Intriguingly, ssDNA binds the MCM ring interior perpendicular to the central channel with defined polarity. In eukaryotes, the MSSB is conserved in several Mcm2-7 subunits, and MSSB mutant combinations in S. cerevisiae Mcm2-7 are not viable. Mutant Mcm2-7 complexes assemble and are recruited to replication origins, but are defective in helicase loading and activation. Our findings identify an important MCM-ssDNA interaction and suggest it functions during helicase activation to select the strand for translocation. DOI: http://dx.doi.org/10.7554/eLife.01993.001. A conserved MCM single-stranded DNA binding element is essential for replication initiation.,Froelich CA, Kang S, Epling LB, Bell SP, Enemark EJ Elife. 2014 Apr 1;3:e01993. doi: 10.7554/eLife.01993. PMID:24692448[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||