1jtt: Difference between revisions

Revision as of 21:54, 2 May 2008

Degenerate interfaces in antigen-antibody complexes

OverviewOverview

In most of the work dealing with the analysis of protein-protein interfaces, a single X-ray structure is available or selected, and implicitly it is assumed that this structure corresponds to the optimal complex for this pair of proteins. However, we have found a degenerate interface in a high-affinity antibody-antigen complex: the two independent complexes of the camel variable domain antibody fragment cAb-Lys3 and its antigen hen egg white lysozyme present in the asymmetric unit of our crystals show a difference in relative orientation between antibody and antigen, leading to important differences at the protein-protein interface. A third cAb-Lys3-hen lysozyme complex in a different crystal form adopts yet another relative orientation. Our results show that protein-protein interface characteristics can vary significantly between different specimens of the same high-affinity antibody-protein antigen complex. Consideration should be given to this type of observation when trying to establish general protein-protein interface characteristics.

About this StructureAbout this Structure

1JTT is a Protein complex structure of sequences from Camelus dromedarius and Gallus gallus. Full crystallographic information is available from OCA.

ReferenceReference

Degenerate interfaces in antigen-antibody complexes., Decanniere K, Transue TR, Desmyter A, Maes D, Muyldermans S, Wyns L, J Mol Biol. 2001 Oct 26;313(3):473-8. PMID:11676532 Page seeded by OCA on Fri May 2 21:54:40 2008

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OCA

@@ Line 1: / Line 1: @@
 [[Image:1jtt.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1jtt |SIZE=350|CAPTION= <scene name='initialview01'>1jtt</scene>, resolution 2.1&Aring;
+The line below this paragraph, containing "STRUCTURE_1jtt", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1jtt|  PDB=1jtt  |  SCENE=  }}
-|RELATEDENTRY=[[1mel|1MEL]], [[1jto|1JTO]], [[1jtp|1JTP]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jtt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jtt OCA], [http://www.ebi.ac.uk/pdbsum/1jtt PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jtt RCSB]</span>
-}}
 '''Degenerate interfaces in antigen-antibody complexes'''
@@ Line 33: / Line 30: @@
 [[Category: Transue, T R.]]
 [[Category: Wyns, L.]]
-[[Category: binding]]
+[[Category: Binding]]
-[[Category: heavy chain antibody]]
+[[Category: Heavy chain antibody]]
-[[Category: immunoglobulin]]
+[[Category: Immunoglobulin]]
-[[Category: interface]]
+[[Category: Interface]]
-[[Category: vhh]]
+[[Category: Vhh]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 21:54:40 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:38:58 2008''