1jra: Difference between revisions

Revision as of 21:44, 2 May 2008

Crystal Structure of Erv2p

OverviewOverview

Erv2p is an FAD-dependent sulfhydryl oxidase that can promote disulfide bond formation during protein biosynthesis in the yeast endoplasmic reticulum. The structure of Erv2p, determined by X-ray crystallography to 1.5 A resolution, reveals a helix-rich dimer with no global resemblance to other known FAD-binding proteins or thiol oxidoreductases. Two pairs of cysteine residues are required for Erv2p activity. The first (Cys-Gly-Glu-Cys) is adjacent to the isoalloxazine ring of the FAD. The second (Cys-Gly-Cys) is part of a flexible C-terminal segment that can swing into the vicinity of the first cysteine pair in the opposite subunit of the dimer and may shuttle electrons between substrate protein dithiols and the FAD-proximal disulfide.

About this StructureAbout this Structure

1JRA is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

A new FAD-binding fold and intersubunit disulfide shuttle in the thiol oxidase Erv2p., Gross E, Sevier CS, Vala A, Kaiser CA, Fass D, Nat Struct Biol. 2002 Jan;9(1):61-7. PMID:11740506 Page seeded by OCA on Fri May 2 21:44:28 2008

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OCA

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 [[Image:1jra.jpg|left|200px]]
- {{Structure
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-|LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>
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-|GENE= ERV2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
+-->
-|DOMAIN=
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-|RELATEDENTRY=[[1jr8|1JR8]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jra FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jra OCA], [http://www.ebi.ac.uk/pdbsum/1jra PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jra RCSB]</span>
-}}
 '''Crystal Structure of Erv2p'''
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 [[Category: Sevier, C S.]]
 [[Category: Vala, A.]]
-[[Category: cxxc]]
+[[Category: Cxxc]]
-[[Category: fad]]
+[[Category: Fad]]
-[[Category: helical bundle]]
+[[Category: Helical bundle]]
-[[Category: sulfhydryl oxidase]]
+[[Category: Sulfhydryl oxidase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 21:44:28 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:37:51 2008''