2l9h: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Oligomeric Structure of the Chemokine CCL5/RANTES from NMR, MS, and SAXS Data== | ==Oligomeric Structure of the Chemokine CCL5/RANTES from NMR, MS, and SAXS Data== | ||
<StructureSection load='2l9h' size='340' side='right'caption='[[2l9h | <StructureSection load='2l9h' size='340' side='right'caption='[[2l9h]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2l9h]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[2l9h]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L9H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L9H FirstGlance]. <br> | ||
</td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l9h OCA], [https://pdbe.org/2l9h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l9h RCSB], [https://www.ebi.ac.uk/pdbsum/2l9h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l9h ProSAT]</span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l9h OCA], [https://pdbe.org/2l9h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l9h RCSB], [https://www.ebi.ac.uk/pdbsum/2l9h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l9h ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/CCL5_HUMAN CCL5_HUMAN] Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.<ref>PMID:16791620</ref> <ref>PMID:1380064</ref> <ref>PMID:8525373</ref> <ref>PMID:9516414</ref> <ref>PMID:15923218</ref> | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 23: | Line 21: | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Handel | [[Category: Handel TM]] | ||
[[Category: Prestegard | [[Category: Prestegard JH]] | ||
[[Category: Sharp | [[Category: Sharp JS]] | ||
[[Category: Wang | [[Category: Wang X]] | ||
[[Category: Watson | [[Category: Watson CM]] | ||
Revision as of 11:27, 18 January 2023
Oligomeric Structure of the Chemokine CCL5/RANTES from NMR, MS, and SAXS DataOligomeric Structure of the Chemokine CCL5/RANTES from NMR, MS, and SAXS Data
Structural highlights
FunctionCCL5_HUMAN Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.[1] [2] [3] [4] [5] Publication Abstract from PubMedCCL5 (RANTES) is a proinflammatory chemokine known to activate leukocytes through its receptor, CCR5. Although the monomeric form of CCL5 is sufficient to cause cell migration in vitro, CCL5's propensity for aggregation is essential for migration in vivo, T cell activation and apoptosis, and HIV entry into cells. However, there is currently no structural information on CCL5 oligomers larger than the canonical CC chemokine dimer. In this study the solution structure of a CCL5 oligomer was investigated using an integrated approach, including NMR residual dipolar couplings to determine allowed relative orientations of the component monomers, SAXS to restrict overall shape, and hydroxyl radical footprinting and NMR cross-saturation experiments to identify interface residues. The resulting model of the CCL5 oligomer provides a basis for explaining the disaggregating effect of E66 and E26 mutations and suggests mechanisms by which glycosaminoglycan binding may promote oligomer formation and facilitate cell migration in vivo. Oligomeric Structure of the Chemokine CCL5/RANTES from NMR, MS, and SAXS Data.,Wang X, Watson C, Sharp JS, Handel TM, Prestegard JH Structure. 2011 Aug 10;19(8):1138-48. PMID:21827949[6] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||