1jmc: Difference between revisions

Revision as of 21:24, 2 May 2008

SINGLE STRANDED DNA-BINDING DOMAIN OF HUMAN REPLICATION PROTEIN A BOUND TO SINGLE STRANDED DNA, RPA70 SUBUNIT, RESIDUES 183-420

OverviewOverview

The single-stranded-DNA-binding proteins (SSBs) are essential for DNA function in prokaryotic and eukaryotic cells, mitochondria, phages and viruses. The structures of four SSBs have been solved, but the molecular details of the interaction of SSBs with DNA remain speculative. We report here the crystal structure at 2.4 A resolution of the single-stranded-DNA-binding domain of human replication protein A (RPA) bound to DNA. Replication protein A is a heterotrimeric SSB that is highly conserved in eukaryotes. The largest subunit, RPA70, binds to single-stranded (ss)DNA and mediates interactions with many cellular and viral proteins. The DNA-binding domain, which lies in the middle of RPA70, comprises two structurally homologous subdomains oriented in tandem. The ssDNA lies in a channel that extends from one subdomain to the other. The structure of each RPA70 subdomain is similar to those of the bacteriophage SSBs, indicating that the mechanism of ssDNA-binding is conserved.

About this StructureAbout this Structure

1JMC is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structure of the single-stranded-DNA-binding domain of replication protein A bound to DNA., Bochkarev A, Pfuetzner RA, Edwards AM, Frappier L, Nature. 1997 Jan 9;385(6612):176-81. PMID:8990123 Page seeded by OCA on Fri May 2 21:24:31 2008

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OCA

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 [[Image:1jmc.gif|left|200px]]
- {{Structure
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 '''SINGLE STRANDED DNA-BINDING DOMAIN OF HUMAN REPLICATION PROTEIN A BOUND TO SINGLE STRANDED DNA, RPA70 SUBUNIT, RESIDUES 183-420'''
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 [[Category: Frappier, L.]]
 [[Category: Pfuetzner, R.]]
-[[Category: complex (dna-binding protein/dna)]]
+[[Category: Protein-ssdna complex]]
-[[Category: human ssdna binding replication protein a(rpa)]]
+[[Category: Single stranded dna-binding protein]]
-[[Category: protein-ssdna complex]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 21:24:31 2008''
-[[Category: single stranded dna-binding protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:35:41 2008''