1hmh: Difference between revisions
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==See Also== | ==See Also== | ||
*[[Ribozyme|Ribozyme]] | *[[Ribozyme 3D structures|Ribozyme 3D structures]] | ||
== References == | == References == | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Flaherty | [[Category: Flaherty KM]] | ||
[[Category: McKay | [[Category: McKay DB]] | ||
[[Category: Pley | [[Category: Pley HW]] | ||
Revision as of 11:03, 11 January 2023
THREE-DIMENSIONAL STRUCTURE OF A HAMMERHEAD RIBOZYMETHREE-DIMENSIONAL STRUCTURE OF A HAMMERHEAD RIBOZYME
Structural highlights
Publication Abstract from PubMedThe hammerhead ribozyme is a small catalytic RNA motif made up of three base-paired stems and a core of highly conserved, non-complementary nucleotides essential for catalysis. The X-ray crystallographic structure of a hammerhead RNA-DNA ribozyme-inhibitor complex at 2.6 A resolution reveals that the base-paired stems are A-form helices and that the core has two structural domains. The first domain is formed by the sequence 5'-CUGA following stem I and is a sharp turn identical to the uridine turn of transfer RNA, whereas the second is a non-Watson-Crick three-base-pair duplex with a divalent-ion binding site. The phosphodiester backbone of the DNA inhibitor strand is splayed out at the phosphate 5' to the cleavage site. The structure indicates that the ribozyme may destabilize a substrate strand in order to facilitate twisting of the substrate to allow cleavage of the scissile bond. Three-dimensional structure of a hammerhead ribozyme.,Pley HW, Flaherty KM, McKay DB Nature. 1994 Nov 3;372(6501):68-74. PMID:7969422[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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