1jjk: Difference between revisions

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[[Image:1jjk.gif|left|200px]]
[[Image:1jjk.gif|left|200px]]


{{Structure
<!--
|PDB= 1jjk |SIZE=350|CAPTION= <scene name='initialview01'>1jjk</scene>, resolution 3.&Aring;
The line below this paragraph, containing "STRUCTURE_1jjk", creates the "Structure Box" on the page.
|SITE=
You may change the PDB parameter (which sets the PDB file loaded into the applet)
|LIGAND= <scene name='pdbligand=BMP:6-HYDROXYURIDINE-5&#39;-PHOSPHATE'>BMP</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Orotidine-5'-phosphate_decarboxylase Orotidine-5'-phosphate decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.23 4.1.1.23] </span>
or leave the SCENE parameter empty for the default display.
|GENE=  
-->
|DOMAIN=
{{STRUCTURE_1jjk| PDB=1jjk  | SCENE= }}  
|RELATEDENTRY=[[1eix|1EIX]]
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jjk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jjk OCA], [http://www.ebi.ac.uk/pdbsum/1jjk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jjk RCSB]</span>
}}


'''Selenomethionine Substitution of Orotidine-5'-monophosphate Decarboxylase from E. coli Causes a Change in Crystal Contacts and Space Group'''
'''Selenomethionine Substitution of Orotidine-5'-monophosphate Decarboxylase from E. coli Causes a Change in Crystal Contacts and Space Group'''
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[[Category: Larsen, S.]]
[[Category: Larsen, S.]]
[[Category: Poulsen, J C.N.]]
[[Category: Poulsen, J C.N.]]
[[Category: alpha-beta-barrel]]
[[Category: Alpha-beta-barrel]]
[[Category: homodimer]]
[[Category: Homodimer]]
[[Category: protein-inhibitor complex]]
[[Category: Protein-inhibitor complex]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 21:18:14 2008''
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:34:39 2008''

Revision as of 21:18, 2 May 2008

File:1jjk.gif

Template:STRUCTURE 1jjk

Selenomethionine Substitution of Orotidine-5'-monophosphate Decarboxylase from E. coli Causes a Change in Crystal Contacts and Space Group


OverviewOverview

Orotidine 5'-monophosphate decarboxylase (ODCase) catalyses the decarboxylation of orotidine 5'-monophosphate to uridine 5'-monophosphate, the last step in the de novo biosynthesis of uridine 5'-monophosphate. In order to determine the structure of ODCase from Escherichia coli by the multi-wavelength anomalous dispersion technique, both native and SeMet-substituted proteins have been produced and purified. During the production of SeMet ODCase, it was observed that SeMet was the only amino acid that it was necessary to add to the defined medium during expression. SeMet-substituted ODCase in complex with the inhibitor 1-(5'-phospho-beta-D-ribofuranosyl)barbituric acid crystallizes under similar conditions as the native enzyme. In contrast to the native enzyme, where the crystals belong to the orthorhombic space group P2(1)2(1)2(1), the SeMet-substituted enzyme crystallizes in the monoclinic space group P2(1), with a quadrupling of the volume of the asymmetric unit. Despite the drastic difference in symmetry, the overall crystal packing is effectively identical in the two crystal forms. The change in space group appears to originate in differences in the crystal contacts near the SeMet and Met residues. These differences can be rationalized in terms of SeMet's larger size and hydrophobicity.

About this StructureAbout this Structure

1JJK is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Selenomethionine substitution of orotidine-5'-monophosphate decarboxylase causes a change in crystal contacts and space group., Poulsen JC, Harris P, Jensen KF, Larsen S, Acta Crystallogr D Biol Crystallogr. 2001 Sep;57(Pt 9):1251-9. Epub 2001, Aug 23. PMID:11526316 Page seeded by OCA on Fri May 2 21:18:14 2008

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