7f0t: Difference between revisions
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==== | ==Cryo-EM structure of dopamine receptor 1 and mini-Gs complex with dopamine bound== | ||
<StructureSection load='7f0t' size='340' side='right'caption='[[7f0t]]' scene=''> | <StructureSection load='7f0t' size='340' side='right'caption='[[7f0t]], [[Resolution|resolution]] 3.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7f0t]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F0T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F0T FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f0t OCA], [https://pdbe.org/7f0t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f0t RCSB], [https://www.ebi.ac.uk/pdbsum/7f0t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f0t ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LDP:L-DOPAMINE'>LDP</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f0t OCA], [https://pdbe.org/7f0t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f0t RCSB], [https://www.ebi.ac.uk/pdbsum/7f0t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f0t ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/DRD1_HUMAN DRD1_HUMAN] Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
G protein-coupled receptors (GPCRs) comprise the largest family of membrane receptors and are the most important drug targets. An agonist-bound GPCR engages heterotrimeric G proteins and triggers the exchange of guanosine diphosphate (GDP) with guanosine triphosphate (GTP) to promote G protein activation. A complete understanding of molecular mechanisms of G protein activation has been hindered by a lack of structural information of GPCR-G protein complex in nucleotide-bound states. Here, we report the cryo-EM structures of the D1 dopamine receptor and mini-G(s) complex in the nucleotide-free and nucleotide-bound states. These structures reveal major conformational changes in Galpha such as structural rearrangements of the carboxyl- and amino-terminal alpha helices that account for the release of GDP and the GTP-dependent dissociation of Galpha from Gbetagamma subunits. As validated by biochemical and cellular signaling studies, our structures shed light into the molecular basis of the entire signaling events of GPCR-mediated G protein activation. | |||
Structural insights into G protein activation by D1 dopamine receptor.,Teng X, Chen S, Wang Q, Chen Z, Wang X, Huang N, Zheng S Sci Adv. 2022 Jun 10;8(23):eabo4158. doi: 10.1126/sciadv.abo4158. Epub 2022 Jun , 10. PMID:35687690<ref>PMID:35687690</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7f0t" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Synthetic construct]] | ||
[[Category: Xiao T]] | |||
[[Category: Zheng S]] | |||