8bd6: Difference between revisions

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-'''Unreleased structure'''
-The entry 8bd6 is ON HOLD
+==Cas12k-sgRNA-dsDNA-TnsC non-productive complex==
+<StructureSection load='8bd6' size='340' side='right'caption='[[8bd6]], [[Resolution|resolution]] 4.10&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8bd6]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Scytonema_hofmannii Scytonema hofmannii] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BD6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BD6 FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8bd6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8bd6 OCA], [https://pdbe.org/8bd6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8bd6 RCSB], [https://www.ebi.ac.uk/pdbsum/8bd6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8bd6 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A8M0FGU0_9CYAN A0A8M0FGU0_9CYAN]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+CRISPR-Cas systems have been co-opted by Tn7-like transposable elements to direct RNA-guided transposition. Type V-K CRISPR-associated transposons rely on the concerted activities of the pseudonuclease Cas12k, the AAA+ ATPase TnsC, the Zn-finger protein TniQ, and the transposase TnsB. Here we present a cryo-electron microscopic structure of a target DNA-bound Cas12k-transposon recruitment complex comprised of RNA-guided Cas12k, TniQ, a polymeric TnsC filament and, unexpectedly, the ribosomal protein S15. Complex assembly, mediated by a network of interactions involving the guide RNA, TniQ, and S15, results in R-loop completion. TniQ contacts two TnsC protomers at the Cas12k-proximal filament end, likely nucleating its polymerization. Transposition activity assays corroborate our structural findings, implying that S15 is a bona fide component of the type V crRNA-guided transposon machinery. Altogether, our work uncovers key mechanistic aspects underpinning RNA-mediated assembly of CRISPR-associated transposons to guide their development as programmable tools for site-specific insertion of large DNA payloads.
-Authors: Schmitz, M., Querques, I., Oberli, S., Chanez, C., Jinek, M.
+Structural basis for the assembly of the type V CRISPR-associated transposon complex.,Schmitz M, Querques I, Oberli S, Chanez C, Jinek M Cell. 2022 Dec 22;185(26):4999-5010.e17. doi: 10.1016/j.cell.2022.11.009. Epub , 2022 Nov 25. PMID:36435179<ref>PMID:36435179</ref>
-Description: Cas12k-sgRNA-dsDNA-TnsC non-productive complex
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Jinek, M]]
+<div class="pdbe-citations 8bd6" style="background-color:#fffaf0;"></div>
-[[Category: Oberli, S]]
+== References ==
-[[Category: Querques, I]]
+<references/>
-[[Category: Schmitz, M]]
+__TOC__
-[[Category: Chanez, C]]
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Scytonema hofmannii]]
+[[Category: Synthetic construct]]
+[[Category: Chanez C]]
+[[Category: Jinek M]]
+[[Category: Oberli S]]
+[[Category: Querques I]]
+[[Category: Schmitz M]]