4me2: Difference between revisions
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==Crystal Structure of THA8 protein from Brachypodium distachyon== | ==Crystal Structure of THA8 protein from Brachypodium distachyon== | ||
<StructureSection load='4me2' size='340' side='right' caption='[[4me2]], [[Resolution|resolution]] 1.60Å' scene=''> | <StructureSection load='4me2' size='340' side='right'caption='[[4me2]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4me2]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4me2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Brachypodium_distachyon Brachypodium distachyon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ME2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ME2 FirstGlance]. <br> | ||
</td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4me2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4me2 OCA], [https://pdbe.org/4me2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4me2 RCSB], [https://www.ebi.ac.uk/pdbsum/4me2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4me2 ProSAT]</span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/I1HB13_BRADI I1HB13_BRADI] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Brachypodium distachyon]] | ||
[[Category: Ban | [[Category: Large Structures]] | ||
[[Category: Brunzelle | [[Category: Ban T]] | ||
[[Category: Chen | [[Category: Brunzelle JS]] | ||
[[Category: Gu | [[Category: Chen RZ]] | ||
[[Category: Ke | [[Category: Gu X]] | ||
[[Category: Melcher | [[Category: Ke J]] | ||
[[Category: Xu | [[Category: Melcher K]] | ||
[[Category: Xu HE]] | |||
Revision as of 14:11, 21 December 2022
Crystal Structure of THA8 protein from Brachypodium distachyonCrystal Structure of THA8 protein from Brachypodium distachyon
Structural highlights
FunctionPublication Abstract from PubMedThylakoid assembly 8 (THA8) is a pentatricopeptide repeat (PPR) RNA-binding protein required for the splicing of the transcript of ycf3, a gene involved in chloroplast thylakoid-membrane biogenesis. Here we report the identification of multiple THA8-binding sites in the ycf3 intron and present crystal structures of Brachypodium distachyon THA8 either free of RNA or bound to two of the identified RNA sites. The apostructure reveals a THA8 monomer with five tandem PPR repeats arranged in a planar fold. The complexes of THA8 bound to the two short RNA fragments surprisingly reveal asymmetric THA8 dimers with the bound RNAs at the dimeric interface. RNA binding induces THA8 dimerization, with a conserved G nucleotide of the bound RNAs making extensive contacts with both monomers. Together, these results establish a new model of RNA recognition by RNA-induced formation of an asymmetric dimer of a PPR protein. Structural basis for RNA recognition by a dimeric PPR-protein complex.,Ke J, Chen RZ, Ban T, Zhou XE, Gu X, Tan MH, Chen C, Kang Y, Brunzelle JS, Zhu JK, Melcher K, Xu HE Nat Struct Mol Biol. 2013 Nov 3. doi: 10.1038/nsmb.2710. PMID:24186060[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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