4mb4: Difference between revisions
Jump to navigation
Jump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Crystal structure of E153Q mutant of cold-adapted chitinase from Moritella complex with Nag4== | ==Crystal structure of E153Q mutant of cold-adapted chitinase from Moritella complex with Nag4== | ||
<StructureSection load='4mb4' size='340' side='right' caption='[[4mb4]], [[Resolution|resolution]] 1.48Å' scene=''> | <StructureSection load='4mb4' size='340' side='right'caption='[[4mb4]], [[Resolution|resolution]] 1.48Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4mb4]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4mb4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Moritella_marina Moritella marina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MB4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MB4 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mb4 OCA], [https://pdbe.org/4mb4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mb4 RCSB], [https://www.ebi.ac.uk/pdbsum/4mb4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mb4 ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/B1VBB0_MORMI B1VBB0_MORMI] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 21: | Line 20: | ||
==See Also== | ==See Also== | ||
*[[Chitinase|Chitinase]] | *[[Chitinase 3D structures|Chitinase 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Moritella marina]] | ||
[[Category: Malecki | [[Category: Malecki PH]] | ||
[[Category: Rypniewski | [[Category: Rypniewski W]] | ||
[[Category: Vorgias | [[Category: Vorgias CE]] | ||
Revision as of 14:07, 21 December 2022
Crystal structure of E153Q mutant of cold-adapted chitinase from Moritella complex with Nag4Crystal structure of E153Q mutant of cold-adapted chitinase from Moritella complex with Nag4
Structural highlights
FunctionPublication Abstract from PubMedThe four-domain structure of chitinase 60 from Moritella marina (MmChi60) is outstanding in its complexity. Many glycoside hydrolases, such as chitinases and cellulases, have multi-domain structures, but only a few have been solved. The flexibility of the hinge regions between the domains apparently makes these proteins difficult to crystallize. The analysis of an active-site mutant of MmChi60 in an unliganded form and in complex with the substrates NAG4 and NAG5 revealed significant differences in the substrate-binding site compared with the previously determined complexes of most studied chitinases. A SAXS experiment demonstrated that in addition to the elongated state found in the crystal, the protein can adapt other conformations in solution ranging from fully extended to compact. Crystal structures of substrate-bound chitinase from the psychrophilic bacterium Moritella marina and its structure in solution.,Malecki PH, Vorgias CE, Petoukhov MV, Svergun DI, Rypniewski W Acta Crystallogr D Biol Crystallogr. 2014 Mar;70(Pt 3):676-84. doi:, 10.1107/S1399004713032264. Epub 2014 Feb 15. PMID:24598737[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||