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==A novel domain in the microcephaly protein CPAP suggests a role in centriole architecture== | ==A novel domain in the microcephaly protein CPAP suggests a role in centriole architecture== | ||
<StructureSection load='4lzf' size='340' side='right' caption='[[4lzf]], [[Resolution|resolution]] 1.72Å' scene=''> | <StructureSection load='4lzf' size='340' side='right'caption='[[4lzf]], [[Resolution|resolution]] 1.72Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4lzf]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4lzf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Danio_rerio Danio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LZF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LZF FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lzf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lzf OCA], [https://pdbe.org/4lzf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lzf RCSB], [https://www.ebi.ac.uk/pdbsum/4lzf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lzf ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/E7FCY1_DANRE E7FCY1_DANRE] | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 24: | Line 22: | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Danio rerio]] | ||
[[Category: Cutts | [[Category: Large Structures]] | ||
[[Category: Erat | [[Category: Cutts E]] | ||
[[Category: Hatzopoulos | [[Category: Erat MC]] | ||
[[Category: Rogala | [[Category: Hatzopoulos GN]] | ||
[[Category: Slatter | [[Category: Rogala K]] | ||
[[Category: Stansfeld | [[Category: Slatter L]] | ||
[[Category: Vakonakis | [[Category: Stansfeld PJ]] | ||
[[Category: Vakonakis I]] | |||
Revision as of 13:42, 21 December 2022
A novel domain in the microcephaly protein CPAP suggests a role in centriole architectureA novel domain in the microcephaly protein CPAP suggests a role in centriole architecture
Structural highlights
FunctionPublication Abstract from PubMedCentrioles are evolutionarily conserved eukaryotic organelles composed of a protein scaffold surrounded by sets of microtubules organized with a 9-fold radial symmetry. CPAP, a centriolar protein essential for microtubule recruitment, features a C-terminal domain of unknown structure, the G-box. A missense mutation in the G-box reduces affinity for the centriolar shuttling protein STIL and causes primary microcephaly. Here, we characterize the molecular architecture of CPAP and determine the G-box structure alone and in complex with a STIL fragment. The G-box comprises a single elongated beta sheet capable of forming supramolecular assemblies. Structural and biophysical studies highlight the conserved nature of the CPAP-STIL complex. We propose that CPAP acts as a horizontal "strut" that joins the centriolar scaffold with microtubules, whereas G-box domains form perpendicular connections. Structural Analysis of the G-Box Domain of the Microcephaly Protein CPAP Suggests a Role in Centriole Architecture.,Hatzopoulos GN, Erat MC, Cutts E, Rogala KB, Slater LM, Stansfeld PJ, Vakonakis I Structure. 2013 Sep 24. pii: S0969-2126(13)00340-7. doi:, 10.1016/j.str.2013.08.019. PMID:24076405[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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