1jf9: Difference between revisions

Revision as of 21:09, 2 May 2008

Crystal Structure of selenocysteine lyase

OverviewOverview

The Escherichia coli NifS CsdB protein is a member of the homodimeric pyridoxal 5'-phosphate (PLP)-dependent family of enzymes. These enzymes are capable of decomposing cysteine or selenocysteine into L-alanine and sulfur or selenium, respectively. E. coli NifS CsdB has a high specificity for L-selenocysteine in comparison to l-cysteine, suggesting a role for this enzyme is selenium metabolism. The 2.0 A crystal structure of E. coli NifS CsdB reveals a high-resolution view of the active site of this enzyme in apo-, persulfide, perselenide, and selenocysteine-bound intermediates, suggesting a mechanism for the stabilization of the enzyme persulfide and perselenide intermediates during catalysis, a necessary intermediate in the formation of sulfur and selenium containing metabolites.

About this StructureAbout this Structure

1JF9 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Analysis of the E. coli NifS CsdB protein at 2.0 A reveals the structural basis for perselenide and persulfide intermediate formation., Lima CD, J Mol Biol. 2002 Feb 1;315(5):1199-208. PMID:11827487 Page seeded by OCA on Fri May 2 21:09:13 2008

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OCA

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 [[Image:1jf9.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1jf9 |SIZE=350|CAPTION= <scene name='initialview01'>1jf9</scene>, resolution 2.&Aring;
+The line below this paragraph, containing "STRUCTURE_1jf9", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=PLP:PYRIDOXAL-5&#39;-PHOSPHATE'>PLP</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Selenocysteine_lyase Selenocysteine lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.4.1.16 4.4.1.16] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= NIFS/CSDB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+-->
-|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG0520 csdA], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=PRK09295 PRK09295]</span>
+{{STRUCTURE_1jf9|  PDB=1jf9  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jf9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jf9 OCA], [http://www.ebi.ac.uk/pdbsum/1jf9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jf9 RCSB]</span>
-}}
 '''Crystal Structure of selenocysteine lyase'''
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 [[Category: Lima, C D.]]
 [[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]]
-[[Category: cysteine]]
+[[Category: Cysteine]]
-[[Category: new york structural genomix research consortium]]
+[[Category: New york structural genomix research consortium]]
-[[Category: nif]]
+[[Category: Nif]]
-[[Category: nysgxrc]]
+[[Category: Nysgxrc]]
-[[Category: protein structure initiative]]
+[[Category: Protein structure initiative]]
-[[Category: psi]]
+[[Category: Psi]]
-[[Category: pursulfide]]
+[[Category: Pursulfide]]
-[[Category: selenocysteine]]
+[[Category: Selenocysteine]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 21:09:13 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:32:48 2008''