1jb7: Difference between revisions

Revision as of 21:00, 2 May 2008

DNA G-Quartets in a 1.86 A Resolution Structure of an Oxytricha nova Telomeric Protein-DNA Complex

OverviewOverview

The Oxytricha nova telomere end binding protein (OnTEBP) recognizes, binds and protects the single-stranded 3'-terminal DNA extension found at the ends of macronuclear chromosomes. The structure of this complex shows that the single strand GGGGTTTTGGGG DNA binds in a deep cleft between the two protein subunits of OnTEBP, adopting a non-helical and irregular conformation. In extending the resolution limit of this structure to 1.86 A, we were surprised to find a G-quartet linked dimer of the GGGGTTTTGGGG DNA also packing within the crystal lattice and interacting with the telomere end binding protein. The G-quartet DNA exhibits the same structure and topology as previously observed in solution by NMR with diagonally crossing d(TTTT) loops at either end of the four-stranded helix. Additionally, the crystal structure reveals clearly visible Na(+), and specific patterns of bound water molecules in the four non-equivalent grooves. Although the G-quartet:protein contact surfaces are modest and might simply represent crystal packing interactions, it is interesting to speculate that the two types of telomeric DNA-protein interactions observed here might both be important in telomere biology.

About this StructureAbout this Structure

1JB7 is a Protein complex structure of sequences from Sterkiella nova. Full crystallographic information is available from OCA.

ReferenceReference

DNA G-quartets in a 1.86 A resolution structure of an Oxytricha nova telomeric protein-DNA complex., Horvath MP, Schultz SC, J Mol Biol. 2001 Jul 6;310(2):367-77. PMID:11428895 Page seeded by OCA on Fri May 2 21:00:28 2008

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OCA

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 [[Image:1jb7.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1jb7", creates the "Structure Box" on the page.
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
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-|GENE= MAC-56A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=200597 Sterkiella nova]), MAC-41A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=200597 Sterkiella nova])
+-->
-|DOMAIN=
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-|RELATEDENTRY=[[1otc|1otc]], [[156d|156d]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jb7 OCA], [http://www.ebi.ac.uk/pdbsum/1jb7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jb7 RCSB]</span>
-}}
 '''DNA G-Quartets in a 1.86 A Resolution Structure of an Oxytricha nova Telomeric Protein-DNA Complex'''
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 [[Category: Horvath, M P.]]
 [[Category: Schultz, S C.]]
-[[Category: dna hydration]]
+[[Category: Dna hydration]]
-[[Category: dna-protein interaction]]
+[[Category: Dna-protein interaction]]
-[[Category: quadruplex dna]]
+[[Category: Quadruplex dna]]
-[[Category: sodium ion]]
+[[Category: Sodium ion]]
-[[Category: telomere-binding protein]]
+[[Category: Telomere-binding protein]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 21:00:28 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:31:05 2008''