1ja2: Difference between revisions

Revision as of 20:58, 2 May 2008

BINDING OF N-ACETYLGLUCOSAMINE TO CHICKEN EGG LYSOZYME: A POWDER DIFFRACTION STUDY

OverviewOverview

The binding of N-acetylglucosamine (NAG) to chicken egg lysozyme (E.C. 3.2.1.17) was investigated by high-resolution X-ray powder diffraction at room temperature. NAG was found to bind to lysozyme in a rapid precipitation preparation with 0.05 M NaCl buffer pH 6.0, but not 0.05 M NaCl buffer pH 5.0. Binding was indicated by significant and readily apparent changes in the diffraction pattern from that of the apo protein precipitated from the same solvent. The location of NAG bound to lysozyme was easily found from a difference Fourier map generated from structure factors extracted during a preliminary combined Rietveld and stereochemical restraint refinement. Full protein and protein-NAG structures were refined with these techniques (R(wp) = 2.22-2.49%, R(p) = 1.79-1.95%, R(F)(2) = 4.95-6.35%) and revealed a binding mode for NAG which differed from that found in an earlier single-crystal study and probably represents a precursor trapped by rapid precipitation.

About this StructureAbout this Structure

1JA2 is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

ReferenceReference

Binding of N-acetylglucosamine to chicken egg lysozyme: a powder diffraction study., Von Dreele RB, Acta Crystallogr D Biol Crystallogr. 2001 Dec;57(Pt 12):1836-42. Epub 2001, Nov 21. PMID:11717496 Page seeded by OCA on Fri May 2 20:58:01 2008

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OCA

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 [[Image:1ja2.jpg|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1ja2", creates the "Structure Box" on the page.
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span>
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-|RELATEDENTRY=[[1ja4|1JA4]], [[1ja6|1JA6]], [[1ja7|1JA7]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ja2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ja2 OCA], [http://www.ebi.ac.uk/pdbsum/1ja2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ja2 RCSB]</span>
-}}
 '''BINDING OF N-ACETYLGLUCOSAMINE TO CHICKEN EGG LYSOZYME: A POWDER DIFFRACTION STUDY'''
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 [[Category: Single protein]]
 [[Category: Dreele, R B.Von.]]
-[[Category: lysozyme]]
+[[Category: Lysozyme]]
-[[Category: powder diffraction]]
+[[Category: Powder diffraction]]
-[[Category: rietveld refinement]]
+[[Category: Rietveld refinement]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 20:58:01 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:30:37 2008''