1cz0: Difference between revisions
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<StructureSection load='1cz0' size='340' side='right'caption='[[1cz0]], [[Resolution|resolution]] 2.10Å' scene=''> | <StructureSection load='1cz0' size='340' side='right'caption='[[1cz0]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1cz0]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CZ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CZ0 FirstGlance]. <br> | <table><tr><td colspan='2'>[[1cz0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CZ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CZ0 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cz0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cz0 OCA], [https://pdbe.org/1cz0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cz0 RCSB], [https://www.ebi.ac.uk/pdbsum/1cz0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cz0 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cz0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cz0 OCA], [https://pdbe.org/1cz0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cz0 RCSB], [https://www.ebi.ac.uk/pdbsum/1cz0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cz0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/PPO1_PHYPO PPO1_PHYPO] Mediates the homing of a group I intron in the ribosomal DNA. Makes a four-base staggered cut in its ribosomal DNA target sequence. | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Physarum polycephalum]] | ||
[[Category: | [[Category: Chevalier B]] | ||
[[Category: Flick KE]] | |||
[[Category: Galburt EA]] | |||
[[Category: Jurica MS]] | |||
[[Category: | [[Category: Stoddard BL]] | ||
[[Category: | |||
[[Category: | |||
[[Category: | |||
Revision as of 11:48, 7 December 2022
INTRON ENCODED HOMING ENDONUCLEASE I-PPOI/DNA COMPLEX LACKING CATALYTIC METAL IONINTRON ENCODED HOMING ENDONUCLEASE I-PPOI/DNA COMPLEX LACKING CATALYTIC METAL ION
Structural highlights
FunctionPPO1_PHYPO Mediates the homing of a group I intron in the ribosomal DNA. Makes a four-base staggered cut in its ribosomal DNA target sequence. Publication Abstract from PubMedA novel mechanism of DNA endonucleolytic cleavage has been visualized for the homing endonuclease I-PpoI by trapping the uncleaved enzyme-substrate complex and comparing it to the previously visualized product complex. This enzyme employs a unique single metal mechanism. A magnesium ion is coordinated by an asparagine residue and two DNA oxygen atoms and stabilizes the phosphoanion transition state and the 3'oxygen leaving group. A hydrolytic water molecule is activated by a histidine residue for an in-line attack on the scissile phosphate. A strained enzyme-substrate-metal complex is formed before cleavage, then relaxed during the reaction. A novel endonuclease mechanism directly visualized for I-PpoI.,Galburt EA, Chevalier B, Tang W, Jurica MS, Flick KE, Monnat RJ Jr, Stoddard BL Nat Struct Biol. 1999 Dec;6(12):1096-9. PMID:10581547[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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