1j87: Difference between revisions

Revision as of 20:54, 2 May 2008

HUMAN HIGH AFFINITY FC RECEPTOR FC(EPSILON)RI(ALPHA), HEXAGONAL CRYSTAL FORM 1

OverviewOverview

We have solved the structure of the human high affinity IgE receptor, Fc epsilon RI alpha, in six different crystal forms, showing the structure in 15 different chemical environments. This database of structures shows no change in the overall shape of the molecule, as the angle between domains 1 and 2 (D1 and D2) varies little across the ensemble. However, the receptor has local conformational variability in the C' strand of D2 and in the BC loop of D1. In every crystal form, a residue inserts between tryptophan residues 87 and 110, mimicking the position of a proline from the IgE ligand. The different crystal forms reveal a distribution of carbohydrates lining the front and back surfaces of the structure. An analysis of crystal contacts in the different forms indicates regions where the molecule interacts with other proteins, and reveals a potential new binding site distal to the IgE binding site. The results of this study point to new directions for the design of molecules to inhibit the interaction of Fc epsilon RI alpha with its natural ligand and thus to prevent a primary step in the allergic response.

About this StructureAbout this Structure

1J87 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

The analysis of the human high affinity IgE receptor Fc epsilon Ri alpha from multiple crystal forms., Garman SC, Sechi S, Kinet JP, Jardetzky TS, J Mol Biol. 2001 Aug 31;311(5):1049-62. PMID:11531339 Page seeded by OCA on Fri May 2 20:54:22 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1j87.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1j87 |SIZE=350|CAPTION= <scene name='initialview01'>1j87</scene>, resolution 3.20&Aring;
+The line below this paragraph, containing "STRUCTURE_1j87", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1j87|  PDB=1j87  |  SCENE=  }}
-|RELATEDENTRY=[[1f2q|1F2Q]], [[1f6a|1F6A]], [[1j86|1J86]], [[1j88|1J88]], [[1j89|1J89]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j87 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j87 OCA], [http://www.ebi.ac.uk/pdbsum/1j87 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1j87 RCSB]</span>
-}}
 '''HUMAN HIGH AFFINITY FC RECEPTOR FC(EPSILON)RI(ALPHA), HEXAGONAL CRYSTAL FORM 1'''
@@ Line 29: / Line 26: @@
 [[Category: Kinet, J P.]]
 [[Category: Sechi, S.]]
-[[Category: fc receptor]]
+[[Category: Fc receptor]]
-[[Category: glycoprotein]]
+[[Category: Glycoprotein]]
-[[Category: ige receptor]]
+[[Category: Ige receptor]]
-[[Category: immune system]]
+[[Category: Immune system]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 20:54:22 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:29:55 2008''