1j81: Difference between revisions

Revision as of 20:54, 2 May 2008

Osmolyte Stabilization of RNase

OverviewOverview

Osmolytes stabilize proteins to thermal and chemical denaturation. We have studied the effects of the osmolytes sarcosine, betaine, trimethylamine-N-oxide, and taurine on the structure and stability of the protein.peptide complex RNase S using x-ray crystallography and titration calorimetry, respectively. The largest degree of stabilization is achieved with 6 m sarcosine, which increases the denaturation temperatures of RNase S and S pro by 24.6 and 17.4 degrees C, respectively, at pH 5 and protects both proteins against tryptic cleavage. Four crystal structures of RNase S in the presence of different osmolytes do not offer any evidence for osmolyte binding to the folded state of the protein or any perturbation in the water structure surrounding the protein. The degree of stabilization in 6 m sarcosine increases with temperature, ranging from -0.52 kcal mol(-1) at 20 degrees C to -5.4 kcal mol(-1) at 60 degrees C. The data support the thesis that osmolytes that stabilize proteins, do so by perturbing unfolded states, which change conformation to a compact, folding competent state in the presence of osmolyte. The increased stabilization thus results from a decrease in conformational entropy of the unfolded state.

About this StructureAbout this Structure

1J81 is a Protein complex structure of sequences from Bos taurus. Full crystallographic information is available from OCA.

ReferenceReference

Osmolytes stabilize ribonuclease S by stabilizing its fragments S protein and S peptide to compact folding-competent states., Ratnaparkhi GS, Varadarajan R, J Biol Chem. 2001 Aug 3;276(31):28789-98. Epub 2001 May 23. PMID:11373282 Page seeded by OCA on Fri May 2 20:54:07 2008

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OCA

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 [[Image:1j81.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1j81 |SIZE=350|CAPTION= <scene name='initialview01'>1j81</scene>, resolution 2.20&Aring;
+The line below this paragraph, containing "STRUCTURE_1j81", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1j81|  PDB=1j81  |  SCENE=  }}
-|RELATEDENTRY=[[ij7z|IJ7Z]], [[1j80|1J80]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j81 OCA], [http://www.ebi.ac.uk/pdbsum/1j81 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1j81 RCSB]</span>
-}}
 '''Osmolyte Stabilization of RNase'''
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 [[Category: Ratnaparkhi, G S.]]
 [[Category: Varadarajan, R.]]
-[[Category: betaine]]
+[[Category: Betaine]]
-[[Category: osmolyte soaking]]
+[[Category: Osmolyte soaking]]
-[[Category: sarcosine]]
+[[Category: Sarcosine]]
-[[Category: taurine]]
+[[Category: Taurine]]
-[[Category: trimethylamine-n-oxide]]
+[[Category: Trimethylamine-n-oxide]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 20:54:07 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:29:51 2008''