1j5j: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1j5j.jpg|left|200px]] | [[Image:1j5j.jpg|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_1j5j", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
| | or leave the SCENE parameter empty for the default display. | ||
| | --> | ||
{{STRUCTURE_1j5j| PDB=1j5j | SCENE= }} | |||
}} | |||
'''Solution structure of HERG-specific scorpion toxin BeKm-1''' | '''Solution structure of HERG-specific scorpion toxin BeKm-1''' | ||
| Line 36: | Line 33: | ||
[[Category: Olesen, S P.]] | [[Category: Olesen, S P.]] | ||
[[Category: Pluzhnikov, K A.]] | [[Category: Pluzhnikov, K A.]] | ||
[[Category: | [[Category: Alpha-beta motif]] | ||
[[Category: | [[Category: Cysteine-knot motif]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:49:54 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 20:49, 2 May 2008
Solution structure of HERG-specific scorpion toxin BeKm-1
OverviewOverview
The scorpion toxin BeKm-1 is unique among a variety of known short scorpion toxins affecting potassium channels in its selective action on ether-a-go-go-related gene (ERG)-type channels. BeKm-1 shares the common molecular scaffold with other short scorpion toxins. The toxin spatial structure resolved by NMR consists of a short alpha-helix and a triple-stranded antiparallel beta-sheet. By toxin mutagenesis study we identified the residues that are important for the binding of BeKm-1 to the human ERG K+ (HERG) channel. The most critical residues (Tyr-11, Lys-18, Arg-20, Lys-23) are located in the alpha-helix and following loop whereas the "traditional" functional site of other short scorpion toxins is formed by residues from the beta-sheet. Thus the unique location of the binding site of BeKm-1 provides its specificity toward the HERG channel.
About this StructureAbout this Structure
1J5J is a Single protein structure of sequence from Mesobuthus eupeus. Full crystallographic information is available from OCA.
ReferenceReference
New binding site on common molecular scaffold provides HERG channel specificity of scorpion toxin BeKm-1., Korolkova YV, Bocharov EV, Angelo K, Maslennikov IV, Grinenko OV, Lipkin AV, Nosyreva ED, Pluzhnikov KA, Olesen SP, Arseniev AS, Grishin EV, J Biol Chem. 2002 Nov 8;277(45):43104-9. Epub 2002 Jul 31. PMID:12151390 Page seeded by OCA on Fri May 2 20:49:54 2008