1j5a: Difference between revisions

Revision as of 20:49, 2 May 2008

STRUCTURAL BASIS FOR THE INTERACTION OF ANTIBIOTICS WITH THE PEPTIDYL TRANSFERASE CENTER IN EUBACTERIA

OverviewOverview

Ribosomes, the site of protein synthesis, are a major target for natural and synthetic antibiotics. Detailed knowledge of antibiotic binding sites is central to understanding the mechanisms of drug action. Conversely, drugs are excellent tools for studying the ribosome function. To elucidate the structural basis of ribosome-antibiotic interactions, we determined the high-resolution X-ray structures of the 50S ribosomal subunit of the eubacterium Deinococcus radiodurans, complexed with the clinically relevant antibiotics chloramphenicol, clindamycin and the three macrolides erythromycin, clarithromycin and roxithromycin. We found that antibiotic binding sites are composed exclusively of segments of 23S ribosomal RNA at the peptidyl transferase cavity and do not involve any interaction of the drugs with ribosomal proteins. Here we report the details of antibiotic interactions with the components of their binding sites. Our results also show the importance of putative Mg+2 ions for the binding of some drugs. This structural analysis should facilitate rational drug design.

About this StructureAbout this Structure

1J5A is a Protein complex structure of sequences from Deinococcus radiodurans. This structure supersedes the now removed PDB entry 1k00. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for the interaction of antibiotics with the peptidyl transferase centre in eubacteria., Schlunzen F, Zarivach R, Harms J, Bashan A, Tocilj A, Albrecht R, Yonath A, Franceschi F, Nature. 2001 Oct 25;413(6858):814-21. PMID:11677599 Page seeded by OCA on Fri May 2 20:49:14 2008

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OCA

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 [[Image:1j5a.gif|left|200px]]
- {{Structure
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-|RELATEDENTRY=[[1jzx|1JZX]], [[1jzy|1JZY]], [[1jzz|1JZZ]], [[1k01|1K01]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j5a OCA], [http://www.ebi.ac.uk/pdbsum/1j5a PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1j5a RCSB]</span>
-}}
 '''STRUCTURAL BASIS FOR THE INTERACTION OF ANTIBIOTICS WITH THE PEPTIDYL TRANSFERASE CENTER IN EUBACTERIA'''
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 ==About this Structure==
-J5A is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. This structure supersedes the now removed PDB entry 1K00. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J5A OCA].
+J5A is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1k00 1k00]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J5A OCA].
 ==Reference==
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 [[Category: 50]]
 [[Category: 5]]
-[[Category: antibiotic]]
+[[Category: Antibiotic]]
-[[Category: clarithromycin]]
+[[Category: Clarithromycin]]
-[[Category: peptidyl transferase center]]
+[[Category: Peptidyl transferase center]]
-[[Category: ribosome]]
+[[Category: Ribosome]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 20:49:14 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:29:03 2008''