4j7b: Difference between revisions
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==Crystal structure of polo-like kinase 1== | ==Crystal structure of polo-like kinase 1== | ||
<StructureSection load='4j7b' size='340' side='right' caption='[[4j7b]], [[Resolution|resolution]] 2.30Å' scene=''> | <StructureSection load='4j7b' size='340' side='right'caption='[[4j7b]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4j7b]] is a 6 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4j7b]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Danio_rerio Danio rerio] and [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J7B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J7B FirstGlance]. <br> | ||
</td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j7b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j7b OCA], [https://pdbe.org/4j7b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j7b RCSB], [https://www.ebi.ac.uk/pdbsum/4j7b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j7b ProSAT]</span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/Q6DRK7_DANRE Q6DRK7_DANRE] | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Danio rerio]] | ||
[[Category: | [[Category: Drosophila melanogaster]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Quan | [[Category: Quan J]] | ||
[[Category: Shen | [[Category: Shen C]] | ||
[[Category: Wang | [[Category: Wang T]] | ||
[[Category: Xu | [[Category: Xu J]] | ||
Revision as of 13:56, 24 November 2022
Crystal structure of polo-like kinase 1Crystal structure of polo-like kinase 1
Structural highlights
FunctionPublication Abstract from PubMedPolo-like kinase 1 (PLK1) is a master regulator of mitosis and is considered a potential drug target for cancer therapy. PLK1 is characterized by an N-terminal kinase domain (KD) and a C-terminal Polo-box domain (PBD). The KD and PBD are mutually inhibited, but the molecular mechanisms of the autoinhibition remain unclear. Here we report the 2.3-A crystal structure of the complex of the Danio rerio KD and PBD together with a PBD-binding motif of Drosophila melanogaster microtubule-associated protein 205 (Map205(PBM)). The structure reveals that the PBD binds and rigidifies the hinge region of the KD in a distinct conformation from that of the phosphopeptide-bound PBD. This structure provides a framework for understanding the autoinhibitory mechanisms of PLK1 and also sheds light on the activation mechanisms of PLK1 by phosphorylation or phosphopeptide binding. Structural basis for the inhibition of Polo-like kinase 1.,Xu J, Shen C, Wang T, Quan J Nat Struct Mol Biol. 2013 Sep;20(9):1047-53. doi: 10.1038/nsmb.2623. Epub 2013, Jul 28. PMID:23893132[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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