1a73: Difference between revisions
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<StructureSection load='1a73' size='340' side='right'caption='[[1a73]], [[Resolution|resolution]] 1.80Å' scene=''> | <StructureSection load='1a73' size='340' side='right'caption='[[1a73]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1a73]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A73 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A73 FirstGlance]. <br> | <table><tr><td colspan='2'>[[1a73]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A73 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A73 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a73 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a73 OCA], [https://pdbe.org/1a73 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a73 RCSB], [https://www.ebi.ac.uk/pdbsum/1a73 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a73 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a73 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a73 OCA], [https://pdbe.org/1a73 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a73 RCSB], [https://www.ebi.ac.uk/pdbsum/1a73 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a73 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/PPO1_PHYPO PPO1_PHYPO] Mediates the homing of a group I intron in the ribosomal DNA. Makes a four-base staggered cut in its ribosomal DNA target sequence. | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Physarum polycephalum]] | ||
[[Category: | [[Category: Flick KE]] | ||
[[Category: | [[Category: Monnat Junior RJ]] | ||
[[Category: | [[Category: Stoddard BL]] | ||
Revision as of 13:45, 24 November 2022
INTRON-ENCODED ENDONUCLEASE I-PPOI COMPLEXED WITH DNAINTRON-ENCODED ENDONUCLEASE I-PPOI COMPLEXED WITH DNA
Structural highlights
FunctionPPO1_PHYPO Mediates the homing of a group I intron in the ribosomal DNA. Makes a four-base staggered cut in its ribosomal DNA target sequence. Publication Abstract from PubMedHoming endonucleases are a diverse collection of proteins that are encoded by genes with mobile, self-splicing introns. They have also been identified in self-splicing inteins (protein introns). These enzymes promote the movement of the DNA sequences that encode them from one chromosome location to another; they do this by making a site-specific double-strand break at a target site in an allele that lacks the corresponding mobile intron. The target sites recognized by these small endonucleases are generally long (14-44 base pairs). Four families of homing endonucleases have been identified, including the LAGLIDADG, the His-Cys box, the GIY-YIG and the H-N-H endonucleases. The first identified His-Cys box homing endonuclease was I-PpoI from the slime mould Physarum polycephalum. Its gene resides in one of only a few nuclear introns known to exhibit genetic mobility. Here we report the structure of the I-PpoI homing endonuclease bound to homing-site DNA determined to 1.8 A resolution. I-PpoI displays an elongated fold of dimensions 25 x 35 x 80 A, with mixed alpha/beta topology. Each I-PpoI monomer contains three antiparallel beta-sheets flanked by two long alpha-helices and a long carboxy-terminal tail, and is stabilized by two bound zinc ions 15 A apart. The enzyme possesses a new zinc-bound fold and endonuclease active site. The structure has been determined in both uncleaved substrate and cleaved product complexes. DNA binding and cleavage by the nuclear intron-encoded homing endonuclease I-PpoI.,Flick KE, Jurica MS, Monnat RJ Jr, Stoddard BL Nature. 1998 Jul 2;394(6688):96-101. PMID:9665136[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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