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==Crystal Structure of a Family GH19 Chitinase (W72A/E67Q mutant) from rye seeds in complex with two (GlcNAc)4 molecules==
==Crystal Structure of a Family GH19 Chitinase (W72A/E67Q mutant) from rye seeds in complex with two (GlcNAc)4 molecules==
<StructureSection load='4j0l' size='340' side='right' caption='[[4j0l]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
<StructureSection load='4j0l' size='340' side='right'caption='[[4j0l]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4j0l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rye Rye]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J0L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J0L FirstGlance]. <br>
<table><tr><td colspan='2'>[[4j0l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Secale_cereale Secale cereale]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J0L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J0L FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rscc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4550 Rye])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j0l OCA], [https://pdbe.org/4j0l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j0l RCSB], [https://www.ebi.ac.uk/pdbsum/4j0l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j0l ProSAT]</span></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j0l OCA], [http://pdbe.org/4j0l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4j0l RCSB], [http://www.ebi.ac.uk/pdbsum/4j0l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4j0l ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/CHIC_SECCE CHIC_SECCE]] Defense against chitin containing fungal pathogens. Binds the hyphal tips of fungi and degrades nascent chitin.<ref>PMID:11999399</ref> <ref>PMID:11826968</ref> <ref>PMID:12092848</ref>
[https://www.uniprot.org/uniprot/CHIC_SECCE CHIC_SECCE] Defense against chitin containing fungal pathogens. Binds the hyphal tips of fungi and degrades nascent chitin.<ref>PMID:11999399</ref> <ref>PMID:11826968</ref> <ref>PMID:12092848</ref>  
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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==See Also==
==See Also==
*[[Chitinase|Chitinase]]
*[[Chitinase 3D structures|Chitinase 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Chitinase]]
[[Category: Large Structures]]
[[Category: Rye]]
[[Category: Secale cereale]]
[[Category: Fukamizo, T]]
[[Category: Fukamizo T]]
[[Category: Numata, T]]
[[Category: Numata T]]
[[Category: Ohnuma, T]]
[[Category: Ohnuma T]]
[[Category: Umemoto, N]]
[[Category: Umemoto N]]
[[Category: Carbohydrate]]
[[Category: Hydrolase]]

Revision as of 00:22, 17 November 2022

Crystal Structure of a Family GH19 Chitinase (W72A/E67Q mutant) from rye seeds in complex with two (GlcNAc)4 moleculesCrystal Structure of a Family GH19 Chitinase (W72A/E67Q mutant) from rye seeds in complex with two (GlcNAc)4 molecules

Structural highlights

4j0l is a 1 chain structure with sequence from Secale cereale. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CHIC_SECCE Defense against chitin containing fungal pathogens. Binds the hyphal tips of fungi and degrades nascent chitin.[1] [2] [3]

Publication Abstract from PubMed

Crystallographic analysis of a mutated form of "loopful" GH19 chitinase from rye seeds a double mutant RSC-c, in which Glu67 and Trp72 are mutated to glutamine and alanine, respectively, (RSC-c-E67Q/W72A) in complex with chitin tetrasaccharide (GlcNAc)(4) revealed that the entire substrate-binding cleft was completely occupied with the sugar residues of two (GlcNAc)(4) molecules. One (GlcNAc)(4) molecule bound to subsites -4 to -1, while the other bound to subsites +1 to +4. Comparisons of the main chain conformation between liganded RSC-c-E67Q/W72A and unliganded wild type RSC-c suggested domain motion essential for catalysis. This is the first report on the complete subsite mapping of GH19 chitinase.

Complete subsite mapping of a "loopful" GH19 chitinase from rye seeds based on its crystal structure.,Ohnuma T, Umemoto N, Kondo K, Numata T, Fukamizo T FEBS Lett. 2013 Aug 19;587(16):2691-7. doi: 10.1016/j.febslet.2013.07.008. Epub, 2013 Jul 18. PMID:23871710[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Ohnuma T, Yagi M, Yamagami T, Taira T, Aso Y, Ishiguro M. Molecular cloning, functional expression, and mutagenesis of cDNA encoding rye (Secale cereale) seed chitinase-c. Biosci Biotechnol Biochem. 2002 Feb;66(2):277-84. PMID:11999399
  2. ↑ Taira T, Yamagami T, Aso Y, Ishiguro M, Ishihara M. Localization, accumulation, and antifungal activity of chitinases in rye (Secale cereale) seed. Biosci Biotechnol Biochem. 2001 Dec;65(12):2710-8. PMID:11826968
  3. ↑ Taira T, Ohnuma T, Yamagami T, Aso Y, Ishiguro M, Ishihara M. Antifungal activity of rye (Secale cereale) seed chitinases: the different binding manner of class I and class II chitinases to the fungal cell walls. Biosci Biotechnol Biochem. 2002 May;66(5):970-7. PMID:12092848
  4. ↑ Ohnuma T, Umemoto N, Kondo K, Numata T, Fukamizo T. Complete subsite mapping of a "loopful" GH19 chitinase from rye seeds based on its crystal structure. FEBS Lett. 2013 Aug 19;587(16):2691-7. doi: 10.1016/j.febslet.2013.07.008. Epub, 2013 Jul 18. PMID:23871710 doi:http://dx.doi.org/10.1016/j.febslet.2013.07.008

4j0l, resolution 1.90Å

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