1j1p: Difference between revisions

Revision as of 20:41, 2 May 2008

Crystal structure of HyHEL-10 Fv mutant LS91A complexed with hen egg white lysozyme

OverviewOverview

To study the role of hydrogen bonding via interfacial water molecules in protein-protein interactions, we examined the interaction between hen egg white lysozyme (HEL) and its HyHEL-10 variable domain fragment (Fv) antibody. We constructed three antibody mutants (l-Y50F, l-S91A, and l-S93A) and investigated the interactions between the mutant Fvs and HEL. Isothermal titration calorimetry indicated that the mutations significantly decreased the negative enthalpy change (8-25 kJ mol(-1)), despite some offset by a favorable entropy change. X-ray crystallography demonstrated that the complexes had nearly identical structures, including the positions of the interfacial water molecules. Taken together, the isothermal titration calorimetric and x-ray crystallographic results indicate that hydrogen bonding via interfacial water enthalpically contributes to the Fv-HEL interaction despite the partial offset because of entropy loss, suggesting that hydrogen bonding stiffens the antigen-antibody complex.

About this StructureAbout this Structure

1J1P is a Protein complex structure of sequences from Gallus gallus and Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

The role of hydrogen bonding via interfacial water molecules in antigen-antibody complexation. The HyHEL-10-HEL interaction., Yokota A, Tsumoto K, Shiroishi M, Kondo H, Kumagai I, J Biol Chem. 2003 Feb 14;278(7):5410-8. Epub 2002 Nov 19. PMID:12444085 Page seeded by OCA on Fri May 2 20:41:50 2008

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OCA

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 [[Image:1j1p.gif|left|200px]]
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-}}
 '''Crystal structure of HyHEL-10 Fv mutant LS91A complexed with hen egg white lysozyme'''
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 [[Category: Tsumoto, K.]]
 [[Category: Yokota, A.]]
-[[Category: antigen-antibody complex]]
+[[Category: Antigen-antibody complex]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 20:41:50 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:27:39 2008''