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==Crystal structure of a green-to-red photoconvertible DRONPA, pcDRONPA in the green-on-state== | ==Crystal structure of a green-to-red photoconvertible DRONPA, pcDRONPA in the green-on-state== | ||
<StructureSection load='4hq8' size='340' side='right' caption='[[4hq8]], [[Resolution|resolution]] 1.95Å' scene=''> | <StructureSection load='4hq8' size='340' side='right'caption='[[4hq8]], [[Resolution|resolution]] 1.95Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4hq8]] is a 6 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4hq8]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Echinophyllia_sp._SC22 Echinophyllia sp. SC22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HQ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HQ8 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CR8:2-[1-AMINO-2-(1H-IMIDAZOL-5-YL)ETHYL]-1-(CARBOXYMETHYL)-4-[(4-OXOCYCLOHEXA-2,5-DIEN-1-YLIDENE)METHYL]-1H-IMIDAZOL-5-OLATE'>CR8</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hq8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hq8 OCA], [https://pdbe.org/4hq8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hq8 RCSB], [https://www.ebi.ac.uk/pdbsum/4hq8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hq8 ProSAT]</span></td></tr> | |||
< | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q5TLG6_9CNID Q5TLG6_9CNID] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Echinophyllia sp. | [[Category: Echinophyllia sp. SC22]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Nguyen Bich N]] | ||
[[Category: | [[Category: Van Meervelt L]] | ||
Revision as of 11:04, 9 November 2022
Crystal structure of a green-to-red photoconvertible DRONPA, pcDRONPA in the green-on-stateCrystal structure of a green-to-red photoconvertible DRONPA, pcDRONPA in the green-on-state
Structural highlights
FunctionPublication Abstract from PubMedAdvanced imaging techniques crucially depend on the labels used. In this work, we present the structure-guided design of a fluorescent protein that displays both reversibly photochromic and green-to-red photoconversion behavior. We first designed ffDronpa, a mutant of the photochromic fluorescent protein Dronpa that matures up to three times faster while retaining its interesting photochromic features. Using a combined evolutionary and structure-driven rational design strategy, we developed a green-to-red photoconvertible ffDronpa mutant, called pcDronpa, and explored different optimization strategies that resulted in its improved version, pcDronpa2. This fluorescent probe combines a high brightness with low photobleaching and photoblinking. We herein show that, despite its tetrameric nature, pcDronpa2 allows for multimodal subdiffraction imaging by sequentially imaging a given sample using both super-resolution fluctuation imaging and localization microscopy. Green-to-Red Photoconvertible Dronpa Mutant for Multimodal Super-resolution Fluorescence Microscopy.,Moeyaert B, Nguyen Bich N, De Zitter E, Rocha S, Clays K, Mizuno H, van Meervelt L, Hofkens J, Dedecker P ACS Nano. 2014 Jan 21. PMID:24410188[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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