1itg: Difference between revisions

Revision as of 20:23, 2 May 2008

CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF HIV-1 INTEGRASE: SIMILARITY TO OTHER POLYNUCLEOTIDYL TRANSFERASES

OverviewOverview

HIV integrase is the enzyme responsible for inserting the viral DNA into the host chromosome; it is essential for HIV replication. The crystal structure of the catalytically active core domain (residues 50 to 212) of HIV-1 integrase was determined at 2.5 A resolution. The central feature of the structure is a five-stranded beta sheet flanked by helical regions. The overall topology reveals that this domain of integrase belongs to a superfamily of polynucleotidyl transferases that includes ribonuclease H and the Holliday junction resolvase RuvC. The active site region is identified by the position of two of the conserved carboxylate residues essential for catalysis, which are located at similar positions in ribonuclease H. In the crystal, two molecules form a dimer with a extensive solvent-inaccessible interface of 1300 A2 per monomer.

About this StructureAbout this Structure

1ITG is a Single protein structure of sequence from Human immunodeficiency virus 1. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the catalytic domain of HIV-1 integrase: similarity to other polynucleotidyl transferases., Dyda F, Hickman AB, Jenkins TM, Engelman A, Craigie R, Davies DR, Science. 1994 Dec 23;266(5193):1981-6. PMID:7801124 Page seeded by OCA on Fri May 2 20:23:38 2008

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OCA

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 [[Image:1itg.gif|left|200px]]
- {{Structure
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-|GENE= HIV-1 INTEGRASE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 Human immunodeficiency virus 1])
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 '''CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF HIV-1 INTEGRASE: SIMILARITY TO OTHER POLYNUCLEOTIDYL TRANSFERASES'''
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 [[Category: Hickman, A B.]]
 [[Category: Jenkins, T M.]]
-[[Category: dna integration]]
+[[Category: Dna integration]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 20:23:38 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:24:23 2008''