1in4: Difference between revisions

Revision as of 20:10, 2 May 2008

THERMOTOGA MARITIMA RUVB HOLLIDAY JUNCTION BRANCH MIGRATION MOTOR

OverviewOverview

The RuvB hexamer is the chemomechanical motor of the RuvAB complex that migrates Holliday junction branch-points in DNA recombination and the rescue of stalled DNA replication forks. The 1.6 A crystal structure of Thermotoga maritima RuvB together with five mutant structures reveal that RuvB is an ATPase-associated with diverse cellular activities (AAA+-class ATPase) with a winged-helix DNA-binding domain. The RuvB-ADP complex structure and mutagenesis suggest how AAA+-class ATPases couple nucleotide binding and hydrolysis to interdomain conformational changes and asymmetry within the RuvB hexamer implied by the crystallographic packing and small-angle X-ray scattering in solution. ATP-driven domain motion is positioned to move double-stranded DNA through the hexamer and drive conformational changes between subunits by altering the complementary hydrophilic protein- protein interfaces. Structural and biochemical analysis of five motifs in the protein suggest that ATP binding is a strained conformation recognized both by sensors and the Walker motifs and that intersubunit activation occurs by an arginine finger motif reminiscent of the GTPase-activating proteins. Taken together, these results provide insights into how RuvB functions as a motor for branch migration of Holliday junctions.

About this StructureAbout this Structure

1IN4 is a Single protein structure of sequence from Thermotoga maritima. Full crystallographic information is available from OCA.

ReferenceReference

Structure and mechanism of the RuvB Holliday junction branch migration motor., Putnam CD, Clancy SB, Tsuruta H, Gonzalez S, Wetmur JG, Tainer JA, J Mol Biol. 2001 Aug 10;311(2):297-310. PMID:11478862 Page seeded by OCA on Fri May 2 20:10:41 2008

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OCA

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 [[Image:1in4.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1in4", creates the "Structure Box" on the page.
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
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-|GENE= RuvB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 Thermotoga maritima])
+-->
-|DOMAIN=
+{{STRUCTURE_1in4|  PDB=1in4  |  SCENE=  }}
-|RELATEDENTRY=[[1in5|1IN5]], [[1in6|1IN6]], [[1in7|1IN7]], [[1in8|1IN8]], [[1j7k|1J7K]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1in4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1in4 OCA], [http://www.ebi.ac.uk/pdbsum/1in4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1in4 RCSB]</span>
-}}
 '''THERMOTOGA MARITIMA RUVB HOLLIDAY JUNCTION BRANCH MIGRATION MOTOR'''
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 [[Category: Putnam, C D.]]
 [[Category: Tainer, J A.]]
-[[Category: aaa+-class atpase]]
+[[Category: Aaa+-class atpase]]
-[[Category: arginine finger]]
+[[Category: Arginine finger]]
-[[Category: atp hydrolysis]]
+[[Category: Atp hydrolysis]]
-[[Category: branch migration]]
+[[Category: Branch migration]]
-[[Category: dna translocase]]
+[[Category: Dna translocase]]
-[[Category: holliday junction]]
+[[Category: Holliday junction]]
-[[Category: sensor 1]]
+[[Category: Sensor 1]]
-[[Category: sensor 2]]
+[[Category: Sensor 2]]
-[[Category: walker some]]
+[[Category: Walker some]]
-[[Category: walker b]]
+[[Category: Walker b]]
-[[Category: winged-helix domain]]
+[[Category: Winged-helix domain]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 20:10:41 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:21:57 2008''