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| <SX load='4f5x' size='340' side='right' viewer='molstar' caption='[[4f5x]], [[Resolution|resolution]] 5.00Å' scene=''> | | <SX load='4f5x' size='340' side='right' viewer='molstar' caption='[[4f5x]], [[Resolution|resolution]] 5.00Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[4f5x]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovine_rotavirus Bovine rotavirus], [http://en.wikipedia.org/wiki/Bovine_rotavirus_a Bovine rotavirus a] and [http://en.wikipedia.org/wiki/Simian_11_rotavirus Simian 11 rotavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F5X OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=4F5X FirstGlance]. <br> | | <table><tr><td colspan='2'>[[4f5x]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovine_rotavirus_A Bovine rotavirus A], [https://en.wikipedia.org/wiki/Bovine_rotavirus_strain_NCDV/G6 Bovine rotavirus strain NCDV/G6] and [https://en.wikipedia.org/wiki/Simian_11_rotavirus_(serotype_3_/_strain_SA11-Patton) Simian 11 rotavirus (serotype 3 / strain SA11-Patton)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F5X FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3kz4|3kz4]], [[2r7s|2r7s]]</td></tr>
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f5x OCA], [https://pdbe.org/4f5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f5x RCSB], [https://www.ebi.ac.uk/pdbsum/4f5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f5x ProSAT]</span></td></tr> |
| <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span></td></tr>
| |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=4f5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f5x OCA], [http://pdbe.org/4f5x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4f5x RCSB], [http://www.ebi.ac.uk/pdbsum/4f5x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4f5x ProSAT]</span></td></tr> | |
| </table> | | </table> |
| == Function == | | == Function == |
| [[http://www.uniprot.org/uniprot/RDRP_ROTSP RDRP_ROTSP]] RNA-directed RNA polymerase that is involved in both transcription and genome replication. Together with VP3 capping enzyme, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. One copy of each of the viral (+)RNAs is also recruited during core assembly, together with newly synthesized polymerase complexes and VP2. The polymerase of these novo-formed particles catalyzes the synthesis of complementary minus-strands leading to dsRNA formation. To do so, the polymerase specifically recognizes and binds 4 bases 5'-UGUG-3' in the conserved 3'-sequence of plus-strand RNA templates. VP2 presumably activates the autoinhibited VP1-RNA complex to coordinate packaging and genome replication. Once dsRNA synthesis is complete, the polymerase switches to the transcriptional mode, thus providing secondary transcription.<ref>PMID:9371626</ref> <ref>PMID:19000820</ref> [[http://www.uniprot.org/uniprot/VP6_ROTBN VP6_ROTBN]] Intermediate capsid protein that self assembles to form an icosahedral capsid with a T=13 symmetry, which consists of 230 trimers of VP6, with channels at each of its five-fold vertices. This capsid constitutes the middle concentric layer of the viral mature particle. The innermost VP2 capsid and the intermediate VP6 capsid remain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nacent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels at the five-fold axes. VP6 is required for the transcription activity of the DLP (By similarity). | | [https://www.uniprot.org/uniprot/H9N1A6_9REOV H9N1A6_9REOV] |
| <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| ==See Also== | | ==See Also== |
| *[[RNA polymerase 3D structures|RNA polymerase 3D structures]] | | *[[RNA polymerase 3D structures|RNA polymerase 3D structures]] |
| | *[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]] |
| == References == | | == References == |
| <references/> | | <references/> |
| __TOC__ | | __TOC__ |
| </SX> | | </SX> |
| [[Category: Bovine rotavirus]] | | [[Category: Bovine rotavirus A]] |
| [[Category: Bovine rotavirus a]] | | [[Category: Bovine rotavirus strain NCDV/G6]] |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: RNA-directed RNA polymerase]]
| | [[Category: Chen JZ]] |
| [[Category: Simian 11 rotavirus]]
| | [[Category: Estrozi LF]] |
| [[Category: Chen, J Z]] | | [[Category: Goret G]] |
| [[Category: Estrozi, L F]] | | [[Category: Grigorieff N]] |
| [[Category: Goret, G]] | | [[Category: Harrison SC]] |
| [[Category: Grigorieff, N]] | | [[Category: McClain B]] |
| [[Category: Harrison, S C]] | | [[Category: Settembre EC]] |
| [[Category: McClain, B]] | | [[Category: Zhang X]] |
| [[Category: Settembre, E C]] | |
| [[Category: Zhang, X]] | |
| [[Category: Beta-jellyroll]]
| |
| [[Category: Polymerase]]
| |
| [[Category: Transcriptase]]
| |
| [[Category: Virus]]
| |