4dt3: Difference between revisions

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==Crystal structure of zinc-charged lysozyme==
==Crystal structure of zinc-charged lysozyme==
<StructureSection load='4dt3' size='340' side='right' caption='[[4dt3]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
<StructureSection load='4dt3' size='340' side='right'caption='[[4dt3]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4dt3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DT3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DT3 FirstGlance]. <br>
<table><tr><td colspan='2'>[[4dt3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DT3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DT3 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LYZ, LYZ1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dt3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dt3 OCA], [https://pdbe.org/4dt3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dt3 RCSB], [https://www.ebi.ac.uk/pdbsum/4dt3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dt3 ProSAT]</span></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dt3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dt3 OCA], [http://pdbe.org/4dt3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4dt3 RCSB], [http://www.ebi.ac.uk/pdbsum/4dt3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4dt3 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
[[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>  
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Chick]]
[[Category: Gallus gallus]]
[[Category: Lysozyme]]
[[Category: Large Structures]]
[[Category: An, Y J]]
[[Category: An YJ]]
[[Category: Cha, S S]]
[[Category: Cha SS]]
[[Category: Jeong, C S]]
[[Category: Jeong CS]]
[[Category: Hydrolase]]

Revision as of 11:37, 21 September 2022

Crystal structure of zinc-charged lysozymeCrystal structure of zinc-charged lysozyme

Structural highlights

4dt3 is a 1 chain structure with sequence from Gallus gallus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.[1]

Publication Abstract from PubMed

Zinc is a suitable metal for anomalous dispersion phasing methods in protein crystallography. Structure determination using zinc anomalous scattering has been almost exclusively limited to proteins with intrinsically bound zinc(s). Here, it is reported that multiple zinc ions can easily be charged onto the surface of proteins with no intrinsic zinc-binding site by using zinc-containing solutions. Zn derivatization of protein surfaces appears to be a largely unnoticed but promising method of protein structure determination.

Experimental phasing using zinc anomalous scattering.,Cha SS, An YJ, Jeong CS, Kim MK, Lee SG, Lee KH, Oh BH Acta Crystallogr D Biol Crystallogr. 2012 Sep;68(Pt 9):1253-8. doi:, 10.1107/S0907444912024420. Epub 2012 Aug 18. PMID:22948927[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Maehashi K, Matano M, Irisawa T, Uchino M, Kashiwagi Y, Watanabe T. Molecular characterization of goose- and chicken-type lysozymes in emu (Dromaius novaehollandiae): evidence for extremely low lysozyme levels in emu egg white. Gene. 2012 Jan 15;492(1):244-9. doi: 10.1016/j.gene.2011.10.021. Epub 2011 Oct, 25. PMID:22044478 doi:10.1016/j.gene.2011.10.021
  2. ↑ Cha SS, An YJ, Jeong CS, Kim MK, Lee SG, Lee KH, Oh BH. Experimental phasing using zinc anomalous scattering. Acta Crystallogr D Biol Crystallogr. 2012 Sep;68(Pt 9):1253-8. doi:, 10.1107/S0907444912024420. Epub 2012 Aug 18. PMID:22948927 doi:10.1107/S0907444912024420

4dt3, resolution 1.80Å

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