8dx4: Difference between revisions
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==Clostridioides difficile R20291 minor pilin - PilW fused with Maltose Binding Protein== | |||
<StructureSection load='8dx4' size='340' side='right'caption='[[8dx4]], [[Resolution|resolution]] 2.49Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8dx4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridioides_difficile_R20291 Clostridioides difficile R20291] and [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DX4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DX4 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dx4 OCA], [https://pdbe.org/8dx4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dx4 RCSB], [https://www.ebi.ac.uk/pdbsum/8dx4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dx4 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[https://www.uniprot.org/uniprot/A0A0H3N4W9_CLODC A0A0H3N4W9_CLODC]] [[https://www.uniprot.org/uniprot/A0A4P1LXE0_SERSF A0A4P1LXE0_SERSF]] Part of the ABC transporter complex MalEFGK involved in maltose/maltodextrin import. Binds maltose and higher maltodextrins.[RuleBase:RU365005] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Clostridioides difficile is a Gram-positive bacillus, which is a frequent cause of gastrointestinal infections triggered by the depletion of the gut microbiome. Because of the frequent recurrence of these infections after antibiotic treatment, mechanisms of C. difficile persistence and recurrence, including biofilm formation, are of increasing interest. Previously, our group and others found that type IV pili, filamentous helical appendages polymerized from protein subunits, promoted microcolony and biofilm formation in C. difficile. In Gram-negative bacteria, the ability of type IV pili to mediate bacterial self-association has been explained through interactions between the pili of adjacent cells, but type IV pili from several Gram-negative species are also required for natural competence through DNA-uptake. Here, we report the ability of two C. difficile pilin subunits, PilJ and PilW, to bind to DNA in vitro, as well as the defects in biofilm formation in the pilJ and pilW gene-interruption mutants. Additionally, we have resolved the X-ray crystal structure of PilW, which we use to model possible structural mechanisms for the formation of C. difficile biofilm through interactions between type IV pili and the DNA of the extracellular matrix. Taken together, our results provide further insight into the relationship between type IV pilus function and biofilm formation in C. difficile and, more broadly, suggest that DNA-recognition by type IV pili and related structures may have functional importance beyond DNA-uptake for natural competence. | |||
Recognition of extracellular DNA by type IV pili promotes biofilm formation by Clostridioides difficile.,Ronish LA, Sidner B, Yu Y, Piepenbrink KH J Biol Chem. 2022 Sep 2:102449. doi: 10.1016/j.jbc.2022.102449. PMID:36064001<ref>PMID:36064001</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 8dx4" style="background-color:#fffaf0;"></div> | ||
[[Category: Piepenbrink | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Clostridioides difficile R20291]] | |||
[[Category: Escherichia coli]] | |||
[[Category: Large Structures]] | |||
[[Category: Piepenbrink K]] | |||
[[Category: Ronish LA]] | |||
Revision as of 10:50, 21 September 2022
Clostridioides difficile R20291 minor pilin - PilW fused with Maltose Binding ProteinClostridioides difficile R20291 minor pilin - PilW fused with Maltose Binding Protein
Structural highlights
Function[A0A0H3N4W9_CLODC] [A0A4P1LXE0_SERSF] Part of the ABC transporter complex MalEFGK involved in maltose/maltodextrin import. Binds maltose and higher maltodextrins.[RuleBase:RU365005] Publication Abstract from PubMedClostridioides difficile is a Gram-positive bacillus, which is a frequent cause of gastrointestinal infections triggered by the depletion of the gut microbiome. Because of the frequent recurrence of these infections after antibiotic treatment, mechanisms of C. difficile persistence and recurrence, including biofilm formation, are of increasing interest. Previously, our group and others found that type IV pili, filamentous helical appendages polymerized from protein subunits, promoted microcolony and biofilm formation in C. difficile. In Gram-negative bacteria, the ability of type IV pili to mediate bacterial self-association has been explained through interactions between the pili of adjacent cells, but type IV pili from several Gram-negative species are also required for natural competence through DNA-uptake. Here, we report the ability of two C. difficile pilin subunits, PilJ and PilW, to bind to DNA in vitro, as well as the defects in biofilm formation in the pilJ and pilW gene-interruption mutants. Additionally, we have resolved the X-ray crystal structure of PilW, which we use to model possible structural mechanisms for the formation of C. difficile biofilm through interactions between type IV pili and the DNA of the extracellular matrix. Taken together, our results provide further insight into the relationship between type IV pilus function and biofilm formation in C. difficile and, more broadly, suggest that DNA-recognition by type IV pili and related structures may have functional importance beyond DNA-uptake for natural competence. Recognition of extracellular DNA by type IV pili promotes biofilm formation by Clostridioides difficile.,Ronish LA, Sidner B, Yu Y, Piepenbrink KH J Biol Chem. 2022 Sep 2:102449. doi: 10.1016/j.jbc.2022.102449. PMID:36064001[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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