4d74: Difference between revisions
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<StructureSection load='4d74' size='340' side='right'caption='[[4d74]], [[Resolution|resolution]] 1.57Å' scene=''> | <StructureSection load='4d74' size='340' side='right'caption='[[4d74]], [[Resolution|resolution]] 1.57Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4d74]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4d74]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Erwinia_amylovora_ATCC_49946 Erwinia amylovora ATCC 49946]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D74 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D74 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d74 OCA], [https://pdbe.org/4d74 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d74 RCSB], [https://www.ebi.ac.uk/pdbsum/4d74 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d74 ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | == Function == | ||
[[ | [[https://www.uniprot.org/uniprot/AMSI_ERWAM AMSI_ERWAM]] May function as a phosphatase required for amylovoran (an exopolysaccharide that functions as a virulence factor) production. | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Erwinia amylovora ATCC 49946]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Benini S]] | |||
[[Category: Benini | [[Category: Caputi L]] | ||
[[Category: Caputi | [[Category: Cianci M]] | ||
[[Category: Cianci | [[Category: Salomone-Stagni M]] | ||
[[Category: Salomone-Stagni | |||
Revision as of 10:50, 14 September 2022
1.57 A crystal structure of erwinia amylovora tyrosine phosphatase amsI1.57 A crystal structure of erwinia amylovora tyrosine phosphatase amsI
Structural highlights
Function[AMSI_ERWAM] May function as a phosphatase required for amylovoran (an exopolysaccharide that functions as a virulence factor) production. Publication Abstract from PubMedAmsI is a low-molecular-weight protein tyrosine phosphatase that regulates the production of amylovoran in the Gram-negative bacterium Erwinia amylovora, a specific pathogen of rosaceous plants such as apple, pear and quince. Amylovoran is an exopolysaccharide that is necessary for successful infection. In order to shed light on AmsI, its structure was solved at 1.57 A resolution at the same pH as its highest measured activity (pH 5.5). In the active site, a water molecule, bridging between the catalytic Arg15 and the reaction-product analogue sulfate, might be representative of the water molecule attacking the phospho-cysteine intermediate in the second step of the reaction mechanism. Characterization and 1.57 A resolution structure of the key fire blight phosphatase AmsI from Erwinia amylovora.,Salomone-Stagni M, Musiani F, Benini S Acta Crystallogr F Struct Biol Commun. 2016 Dec 1;72(Pt 12):903-910. Epub 2016, Nov 30. PMID:27917839[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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