4cte: Difference between revisions
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<StructureSection load='4cte' size='340' side='right'caption='[[4cte]], [[Resolution|resolution]] 1.80Å' scene=''> | <StructureSection load='4cte' size='340' side='right'caption='[[4cte]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4cte]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4cte]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Zobellia_galactanivorans Zobellia galactanivorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CTE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CTE FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GS1:1-THIO-BETA-D-GLUCOPYRANOSE'>GS1</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GS1:1-THIO-BETA-D-GLUCOPYRANOSE'>GS1</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cte FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cte OCA], [https://pdbe.org/4cte PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cte RCSB], [https://www.ebi.ac.uk/pdbsum/4cte PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cte ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[[https://www.uniprot.org/uniprot/G0L2L9_ZOBGA G0L2L9_ZOBGA]] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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==See Also== | ==See Also== | ||
*[[Glucanase|Glucanase]] | *[[Glucanase 3D structures|Glucanase 3D structures]] | ||
*[[Laminarase 3D structures|Laminarase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Czjzek | [[Category: Zobellia galactanivorans]] | ||
[[Category: Ferrieres | [[Category: Czjzek M]] | ||
[[Category: Ficko-Blean | [[Category: Ferrieres V]] | ||
[[Category: Hehemann | [[Category: Ficko-Blean E]] | ||
[[Category: Jam | [[Category: Hehemann JH]] | ||
[[Category: Labourel | [[Category: Jam M]] | ||
[[Category: Legentil | [[Category: Labourel A]] | ||
[[Category: Michel | [[Category: Legentil L]] | ||
[[Category: Sylla | [[Category: Michel G]] | ||
[[Category: Sylla B]] | |||
Revision as of 10:32, 14 September 2022
Crystal structure of the catalytic domain of the modular laminarinase ZgLamC mutant E142S in complex with a thio-oligosaccharideCrystal structure of the catalytic domain of the modular laminarinase ZgLamC mutant E142S in complex with a thio-oligosaccharide
Structural highlights
FunctionPublication Abstract from PubMedLaminarin is a beta-1,3-D-glucan displaying occasional beta-1,6 branches. This storage polysaccharide of brown algae constitutes an abundant source of carbon for marine bacteria such as Zobellia galactanivorans. This marine member of the Bacteroidetes possesses five putative beta-1,3-glucanases [four belonging to glycosyl hydrolase family 16 (GH16) and one to GH64] with various modular architectures. Here, the characterization of the beta-glucanase ZgLamC is reported. The catalytic GH16 module (ZgLamCGH16) was produced in Escherichia coli and purified. This recombinant enzyme has a preferential specificity for laminarin but also a significant activity on mixed-linked glucan (MLG). The structure of an inactive mutant of ZgLamCGH16 in complex with a thio-beta-1,3-hexaglucan substrate unravelled a straight active-site cleft with three additional pockets flanking subsites -1, -2 and -3. These lateral pockets are occupied by a glycerol, an acetate ion and a chloride ion, respectively. The presence of these molecules in the vicinity of the O6 hydroxyl group of each glucose moiety suggests that ZgLamCGH16 accommodates branched laminarins as substrates. Altogether, ZgLamC is a secreted laminarinase that is likely to be involved in the initial step of degradation of branched laminarin, while the previously characterized ZgLamA efficiently degrades unbranched laminarin and oligo-laminarins. Structural and biochemical characterization of the laminarinase ZgLamCGH16 from Zobellia galactanivorans suggests preferred recognition of branched laminarin.,Labourel A, Jam M, Legentil L, Sylla B, Hehemann JH, Ferrieres V, Czjzek M, Michel G Acta Crystallogr D Biol Crystallogr. 2015 Feb;71(Pt 2):173-84. doi:, 10.1107/S139900471402450X. Epub 2015 Jan 23. PMID:25664729[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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