1idq: Difference between revisions

Revision as of 19:53, 2 May 2008

CRYSTAL STRUCTURE OF NATIVE VANADIUM-CONTAINING CHLOROPEROXIDASE FROM CURVULARIA INAEQUALIS

OverviewOverview

Implications for the catalytic mechanism of the vanadium-containing chloroperoxidase from the fungus Curvularia inaequalis have been obtained from the crystal structures of the native and peroxide forms of the enzyme.The X-ray structures have been solved by difference Fourier techniques using the atomic model of the azide chloroperoxidase complex. The 2.03 A crystal structure (R = 19.7%) of the native enzyme reveals the geometry of the intact catalytic vanadium center. The vanadium is coordinated by four non-protein oxygen atoms and one nitrogen (NE2) atom from histidine 496 in a trigonal bipyramidal fashion. Three oxygens are in the equatorial plane and the fourth oxygen and the nitrogen are at the apexes of the bipyramid. In the 2.24 A crystal structure (R = 17.7%) of the peroxide derivate the peroxide is bound to the vanadium in an eta2-fashion after the release of the apical oxygen ligand. The vanadium is coordinated also by 4 non-protein oxygen atoms and one nitrogen (NE2) from histidine 496. The coordination geometry around the vanadium is that of a distorted tetragonal pyramid with the two peroxide oxygens, one oxygen and the nitrogen in the basal plane and one oxygen in the apical position. A mechanism for the catalytic cycle has been proposed based on these X-ray structures and kinetic data.

About this StructureAbout this Structure

1IDQ is a Single protein structure of sequence from Curvularia inaequalis. Full crystallographic information is available from OCA.

ReferenceReference

Implications for the catalytic mechanism of the vanadium-containing enzyme chloroperoxidase from the fungus Curvularia inaequalis by X-ray structures of the native and peroxide form., Messerschmidt A, Prade L, Wever R, Biol Chem. 1997 Mar-Apr;378(3-4):309-15. PMID:9165086 Page seeded by OCA on Fri May 2 19:53:10 2008

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OCA

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 [[Image:1idq.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1idq", creates the "Structure Box" on the page.
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-|LIGAND= <scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene>
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Chloride_peroxidase Chloride peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.10 1.11.1.10] </span>
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-|RELATEDENTRY=[[1idu|1IDU]], [[1vnc|1VNC]], [[1vne|1VNE]], [[1vnf|1VNF]], [[1vng|1VNG]], [[1vnh|1VNH]], [[1vni|1VNI]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1idq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1idq OCA], [http://www.ebi.ac.uk/pdbsum/1idq PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1idq RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF NATIVE VANADIUM-CONTAINING CHLOROPEROXIDASE FROM CURVULARIA INAEQUALIS'''
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 [[Category: Prade, L.]]
 [[Category: Wever, R.]]
-[[Category: two four-helix bundle]]
+[[Category: Two four-helix bundle]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 19:53:10 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:18:21 2008''