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==Room-temperature structure of lysozyme by serial femtosecond crystallography (BITS)== | ==Room-temperature structure of lysozyme by serial femtosecond crystallography (BITS)== | ||
<StructureSection load='7wuc' size='340' side='right'caption='[[7wuc]]' scene=''> | <StructureSection load='7wuc' size='340' side='right'caption='[[7wuc]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WUC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WUC FirstGlance]. <br> | <table><tr><td colspan='2'>[[7wuc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WUC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WUC FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wuc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wuc OCA], [https://pdbe.org/7wuc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wuc RCSB], [https://www.ebi.ac.uk/pdbsum/7wuc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wuc ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wuc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wuc OCA], [https://pdbe.org/7wuc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wuc RCSB], [https://www.ebi.ac.uk/pdbsum/7wuc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wuc ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Serial femtosecond crystallography (SFX) enables the determination of room-temperature crystal structures of macromolecules with minimized radiation damage and provides time-resolved molecular dynamics by pump-probe or mix-and-inject experiments. In SFX, a variety of sample delivery methods with unique advantages have been developed and applied. The combination of existing sample delivery methods can enable a new approach to SFX data collection that combines the advantages of the individual methods. This study introduces a combined inject-and-transfer system (BITS) method for sample delivery in SFX experiments: a hybrid injection and fixed-target scanning method. BITS allows for solution samples to be reliably deposited on ultraviolet ozone (UVO)-treated polyimide films, at a minimum flow rate of 0.5 nl min(-1), in both vertical and horizontal scanning modes. To utilize BITS in SFX experiments, lysozyme crystal samples were embedded in a viscous lard medium and injected at flow rates of 50-100 nl min(-1) through a syringe needle onto a UVO-treated polyimide film, which was mounted on a fixed-target scan stage. The crystal samples deposited on the film were raster scanned with an X-ray free electron laser using a motion stage in both horizontal and vertical directions. Using the BITS method, the room-temperature structure of lysozyme was successfully determined at a resolution of 2.1 A, and thus BITS could be utilized in future SFX experiments. | |||
Combination of an inject-and-transfer system for serial femtosecond crystallography.,Lee K, Kim J, Baek S, Park J, Park S, Lee JL, Chung WK, Cho Y, Nam KH J Appl Crystallogr. 2022 Jul 5;55(Pt 4):813-822. doi: 10.1107/S1600576722005556. , eCollection 2022 Aug 1. PMID:35979068<ref>PMID:35979068</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7wuc" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Gallus gallus]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Nam KH]] | [[Category: Nam KH]] | ||
Revision as of 10:05, 14 September 2022
Room-temperature structure of lysozyme by serial femtosecond crystallography (BITS)Room-temperature structure of lysozyme by serial femtosecond crystallography (BITS)
Structural highlights
Function[LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.[1] Publication Abstract from PubMedSerial femtosecond crystallography (SFX) enables the determination of room-temperature crystal structures of macromolecules with minimized radiation damage and provides time-resolved molecular dynamics by pump-probe or mix-and-inject experiments. In SFX, a variety of sample delivery methods with unique advantages have been developed and applied. The combination of existing sample delivery methods can enable a new approach to SFX data collection that combines the advantages of the individual methods. This study introduces a combined inject-and-transfer system (BITS) method for sample delivery in SFX experiments: a hybrid injection and fixed-target scanning method. BITS allows for solution samples to be reliably deposited on ultraviolet ozone (UVO)-treated polyimide films, at a minimum flow rate of 0.5 nl min(-1), in both vertical and horizontal scanning modes. To utilize BITS in SFX experiments, lysozyme crystal samples were embedded in a viscous lard medium and injected at flow rates of 50-100 nl min(-1) through a syringe needle onto a UVO-treated polyimide film, which was mounted on a fixed-target scan stage. The crystal samples deposited on the film were raster scanned with an X-ray free electron laser using a motion stage in both horizontal and vertical directions. Using the BITS method, the room-temperature structure of lysozyme was successfully determined at a resolution of 2.1 A, and thus BITS could be utilized in future SFX experiments. Combination of an inject-and-transfer system for serial femtosecond crystallography.,Lee K, Kim J, Baek S, Park J, Park S, Lee JL, Chung WK, Cho Y, Nam KH J Appl Crystallogr. 2022 Jul 5;55(Pt 4):813-822. doi: 10.1107/S1600576722005556. , eCollection 2022 Aug 1. PMID:35979068[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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