1ibk: Difference between revisions

Revision as of 19:48, 2 May 2008

STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH THE ANTIBIOTIC PAROMOMYCIN

OverviewOverview

Crystal structures of the 30S ribosomal subunit in complex with messenger RNA and cognate transfer RNA in the A site, both in the presence and absence of the antibiotic paromomycin, have been solved at between 3.1 and 3.3 angstroms resolution. Cognate transfer RNA (tRNA) binding induces global domain movements of the 30S subunit and changes in the conformation of the universally conserved and essential bases A1492, A1493, and G530 of 16S RNA. These bases interact intimately with the minor groove of the first two base pairs between the codon and anticodon, thus sensing Watson-Crick base-pairing geometry and discriminating against near-cognate tRNA. The third, or "wobble," position of the codon is free to accommodate certain noncanonical base pairs. By partially inducing these structural changes, paromomycin facilitates binding of near-cognate tRNAs.

About this StructureAbout this Structure

1IBK is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.

ReferenceReference

Recognition of cognate transfer RNA by the 30S ribosomal subunit., Ogle JM, Brodersen DE, Clemons WM Jr, Tarry MJ, Carter AP, Ramakrishnan V, Science. 2001 May 4;292(5518):897-902. PMID:11340196 Page seeded by OCA on Fri May 2 19:48:38 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1ibk.gif|left|200px]]
- {{Structure
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-|RELATEDENTRY=[[1fjf|1FJF]], [[1fjg|1FJG]], [[1ibl|1IBL]], [[1ibm|1IBM]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ibk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ibk OCA], [http://www.ebi.ac.uk/pdbsum/1ibk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ibk RCSB]</span>
-}}
 '''STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH THE ANTIBIOTIC PAROMOMYCIN'''
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 [[Category: Tarry, M J.]]
 [[Category: 30s ribosomal subunit]]
-[[Category: aminoglycoside]]
+[[Category: Aminoglycoside]]
-[[Category: antibiotic]]
+[[Category: Antibiotic]]
-[[Category: decoding]]
+[[Category: Decoding]]
-[[Category: paromomycin]]
+[[Category: Paromomycin]]
-[[Category: ribosome]]
+[[Category: Ribosome]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 19:48:38 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:17:24 2008''