1ib1: Difference between revisions

Revision as of 19:47, 2 May 2008

CRYSTAL STRUCTURE OF THE 14-3-3 ZETA:SEROTONIN N-ACETYLTRANSFERASE COMPLEX

OverviewOverview

Serotonin N-acetyltransferase (AANAT) controls the daily rhythm in melatonin synthesis. When isolated from tissue, AANAT copurifies with isoforms epsilon and zeta of 14-3-3. We have determined the structure of AANAT bound to 14-3-3zeta, an association that is phosphorylation dependent. AANAT is bound in the central channel of the 14-3-3zeta dimer, and is held in place by extensive interactions both with the amphipathic phosphopeptide binding groove of 14-3-3zeta and with other parts of the central channel. Thermodynamic and activity measurements, together with crystallographic analysis, indicate that binding of AANAT by 14-3-3zeta modulates AANAT's activity and affinity for its substrates by stabilizing a region of AANAT involved in substrate binding.

About this StructureAbout this Structure

1IB1 is a Protein complex structure of sequences from Homo sapiens and Ovis aries. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the 14-3-3zeta:serotonin N-acetyltransferase complex. a role for scaffolding in enzyme regulation., Obsil T, Ghirlando R, Klein DC, Ganguly S, Dyda F, Cell. 2001 Apr 20;105(2):257-67. PMID:11336675 Page seeded by OCA on Fri May 2 19:47:34 2008

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OCA

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 [[Image:1ib1.gif|left|200px]]
- {{Structure
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Aralkylamine_N-acetyltransferase Aralkylamine N-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.87 2.3.1.87] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= YWHAZ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), AANAT OR SNAT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9940 Ovis aries])
+-->
-|DOMAIN=
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ib1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ib1 OCA], [http://www.ebi.ac.uk/pdbsum/1ib1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ib1 RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF THE 14-3-3 ZETA:SEROTONIN N-ACETYLTRANSFERASE COMPLEX'''
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 [[Category: Obsil, T.]]
 [[Category: 14-3-3]]
-[[Category: n-acetyl transferase]]
+[[Category: N-acetyl transferase]]
-[[Category: phosphorylation]]
+[[Category: Phosphorylation]]
-[[Category: protein-protein complex]]
+[[Category: Protein-protein complex]]
-[[Category: signal transduction]]
+[[Category: Signal transduction]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 19:47:34 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:17:11 2008''