7z10: Difference between revisions
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==== | ==Monomeric respiratory complex IV isolated from S. cerevisiae== | ||
<StructureSection load='7z10' size='340' side='right'caption='[[7z10]]' scene=''> | <StructureSection load='7z10' size='340' side='right'caption='[[7z10]], [[Resolution|resolution]] 3.87Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7z10]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z10 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z10 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z10 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z10 OCA], [https://pdbe.org/7z10 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z10 RCSB], [https://www.ebi.ac.uk/pdbsum/7z10 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z10 ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=CUA:DINUCLEAR+COPPER+ION'>CUA</scene>, <scene name='pdbligand=HEA:HEME-A'>HEA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEF:DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE'>PEF</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z10 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z10 OCA], [https://pdbe.org/7z10 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z10 RCSB], [https://www.ebi.ac.uk/pdbsum/7z10 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z10 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[[https://www.uniprot.org/uniprot/COX9_YEAST COX9_YEAST]] This small integral protein plays a role in holoenzyme assembly or stability. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In mitochondria, complex IV (CIV) can be found as a monomer, a dimer or in association with other respiratory complexes. The atomic structure of the yeast S. cerevisiae CIV in a supercomplex (SC) with complex III (CIII) pointed to a region of significant conformational changes compared to the homologous mammalian CIV structures. These changes involved the matrix side domain of Cox5A at the CIII-CIV interface, and it was suggested that it could be required for SC formation. To investigate this, we solved the structure of the isolated monomeric CIV from S. cerevisiae stabilised in amphipol A8-35 at 3.9 A using cryo-electron microscopy. Only a minor change in flexibility was seen in this Cox5A region, ruling out large CIV conformational shift for interaction with CIII and confirming the different fold of the yeast Cox5A subunit compared to mammalian homologues. Other differences in structure were the absence of two canonical subunits, Cox12 and Cox13, as well as Cox26, which is unique to the yeast CIV. Their absence is most likely due to the protein purification protocol used to isolate CIV from the III-IV SC. | |||
Cryo-EM structure of a monomeric yeast S. cerevisiae complex IV isolated with maltosides: Implications in supercomplex formation.,Ing G, Hartley AM, Pinotsis N, Marechal A Biochim Biophys Acta Bioenerg. 2022 Oct 1;1863(7):148591. doi:, 10.1016/j.bbabio.2022.148591. Epub 2022 Jul 14. PMID:35839926<ref>PMID:35839926</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7z10" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Saccharomyces cerevisiae S288C]] | ||
[[Category: Hartley A]] | |||
[[Category: Ing G]] | |||
[[Category: Marechal A]] | |||
[[Category: Pinotsis N]] | |||