1i96: Difference between revisions

Revision as of 19:43, 2 May 2008

CRYSTAL STRUCTURE OF THE 30S RIBOSOMAL SUBUNIT FROM THERMUS THERMOPHILUS IN COMPLEX WITH THE TRANSLATION INITIATION FACTOR IF3 (C-TERMINAL DOMAIN)

OverviewOverview

The small ribosomal subunit is responsible for the decoding of genetic information and plays a key role in the initiation of protein synthesis. We analyzed by X-ray crystallography the structures of three different complexes of the small ribosomal subunit of Thermus thermophilus with the A-site inhibitor tetracycline, the universal initiation inhibitor edeine and the C-terminal domain of the translation initiation factor IF3. The crystal structure analysis of the complex with tetracycline revealed the functionally important site responsible for the blockage of the A-site. Five additional tetracycline sites resolve most of the controversial biochemical data on the location of tetracycline. The interaction of edeine with the small subunit indicates its role in inhibiting initiation and shows its involvement with P-site tRNA. The location of the C-terminal domain of IF3, at the solvent side of the platform, sheds light on the formation of the initiation complex, and implies that the anti-association activity of IF3 is due to its influence on the conformational dynamics of the small ribosomal subunit.

About this StructureAbout this Structure

1I96 is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structures of complexes of the small ribosomal subunit with tetracycline, edeine and IF3., Pioletti M, Schlunzen F, Harms J, Zarivach R, Gluhmann M, Avila H, Bashan A, Bartels H, Auerbach T, Jacobi C, Hartsch T, Yonath A, Franceschi F, EMBO J. 2001 Apr 17;20(8):1829-39. PMID:11296217 Page seeded by OCA on Fri May 2 19:43:39 2008

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OCA

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 [[Image:1i96.gif|left|200px]]
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-|RELATEDENTRY=[[1fka|1FKA]], [[1tig|1TIG]], [[1i94|1I94]], [[1i95|1I95]], [[1i97|1I97]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i96 OCA], [http://www.ebi.ac.uk/pdbsum/1i96 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1i96 RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF THE 30S RIBOSOMAL SUBUNIT FROM THERMUS THERMOPHILUS IN COMPLEX WITH THE TRANSLATION INITIATION FACTOR IF3 (C-TERMINAL DOMAIN)'''
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 [[Category: Zarivach, R.]]
 [[Category: 30s ribosome]]
-[[Category: if3]]
+[[Category: If3]]
-[[Category: translation initiation factor]]
+[[Category: Translation initiation factor]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 19:43:39 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:16:25 2008''