4c4w: Difference between revisions

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<StructureSection load='4c4w' size='340' side='right'caption='[[4c4w]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
<StructureSection load='4c4w' size='340' side='right'caption='[[4c4w]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4c4w]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Arcfu Arcfu] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C4W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C4W FirstGlance]. <br>
<table><tr><td colspan='2'>[[4c4w]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Kneallhazia_solenopsae Kneallhazia solenopsae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C4W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4C4W FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2HP:DIHYDROGENPHOSPHATE+ION'>2HP</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2HP:DIHYDROGENPHOSPHATE+ION'>2HP</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4c40|4c40]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4c4w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c4w OCA], [https://pdbe.org/4c4w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4c4w RCSB], [https://www.ebi.ac.uk/pdbsum/4c4w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4c4w ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c4w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c4w OCA], [http://pdbe.org/4c4w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4c4w RCSB], [http://www.ebi.ac.uk/pdbsum/4c4w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4c4w ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/SNRPA_HUMAN SNRPA_HUMAN]] Binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. Binds preferentially to the 5'-UGCAC-3' motif in vitro.<ref>PMID:9848648</ref>  [[http://www.uniprot.org/uniprot/RL7A_ARCFU RL7A_ARCFU]] Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, box H/ACA and box C/D sRNAs.<ref>PMID:12560482</ref> 
[[https://www.uniprot.org/uniprot/SNRPA_HUMAN SNRPA_HUMAN]] Binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. Binds preferentially to the 5'-UGCAC-3' motif in vitro.<ref>PMID:9848648</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Kt-23 from Thelohania solenopsae is a rare RNA kink turn (k-turn) where an adenine replaces the normal guanine at the 2n position. L7Ae is a member of a strongly conserved family of proteins that bind a range of k-turn structures in the ribosome, box C/D and H/ACA small nucleolar RNAs and U4 small nuclear RNA. We have solved the crystal structure of T. solenopsae Kt-23 RNA bound to Archeoglobus fulgidus L7Ae protein at a resolution of 2.95 A. The protein binds in the major groove displayed on the outer face of the k-turn, in a manner similar to complexes with standard k-turn structures. The k-turn adopts a standard N3 class conformation, with a single hydrogen bond from A2b N6 to A2n N3. This contrasts with the structure of the same sequence located in the SAM-I riboswitch, where it adopts an N1 structure, showing the inherent plasticity of k-turn structure. This potentially can affect any tertiary interactions in which the RNA participates.
 
Structure of a rare non-standard sequence k-turn bound by L7Ae protein.,Huang L, Lilley DM Nucleic Acids Res. 2014 Jan 29. PMID:24482444<ref>PMID:24482444</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4c4w" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Nucleoprotein|Nucleoprotein]]
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Arcfu]]
[[Category: Archaeoglobus fulgidus DSM 4304]]
[[Category: Human]]
[[Category: Homo sapiens]]
[[Category: Kneallhazia solenopsae]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Huang, L]]
[[Category: Huang L]]
[[Category: Lilley, D M.J]]
[[Category: Lilley DMJ]]
[[Category: Kink turn]]
[[Category: Rna binding protein]]
[[Category: Rna binding protein-rna complex]]

Revision as of 20:27, 7 September 2022

Structure of a rare, non-standard sequence k-turn bound by L7Ae proteinStructure of a rare, non-standard sequence k-turn bound by L7Ae protein

Structural highlights

4c4w is a 8 chain structure with sequence from Archaeoglobus fulgidus DSM 4304, Homo sapiens and Kneallhazia solenopsae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SNRPA_HUMAN] Binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. Binds preferentially to the 5'-UGCAC-3' motif in vitro.[1]

See Also

References

  1. ↑ Lutz CS, Cooke C, O'Connor JP, Kobayashi R, Alwine JC. The snRNP-free U1A (SF-A) complex(es): identification of the largest subunit as PSF, the polypyrimidine-tract binding protein-associated splicing factor. RNA. 1998 Dec;4(12):1493-9. PMID:9848648

4c4w, resolution 2.95Å

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