1i6v: Difference between revisions

Revision as of 19:38, 2 May 2008

THERMUS AQUATICUS CORE RNA POLYMERASE-RIFAMPICIN COMPLEX

OverviewOverview

Rifampicin (Rif) is one of the most potent and broad spectrum antibiotics against bacterial pathogens and is a key component of anti-tuberculosis therapy, stemming from its inhibition of the bacterial RNA polymerase (RNAP). We determined the crystal structure of Thermus aquaticus core RNAP complexed with Rif. The inhibitor binds in a pocket of the RNAP beta subunit deep within the DNA/RNA channel, but more than 12 A away from the active site. The structure, combined with biochemical results, explains the effects of Rif on RNAP function and indicates that the inhibitor acts by directly blocking the path of the elongating RNA when the transcript becomes 2 to 3 nt in length.

About this StructureAbout this Structure

1I6V is a Protein complex structure of sequences from Thermus aquaticus. Full crystallographic information is available from OCA.

ReferenceReference

Structural mechanism for rifampicin inhibition of bacterial rna polymerase., Campbell EA, Korzheva N, Mustaev A, Murakami K, Nair S, Goldfarb A, Darst SA, Cell. 2001 Mar 23;104(6):901-12. PMID:11290327 Page seeded by OCA on Fri May 2 19:38:35 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1i6v.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1i6v |SIZE=350|CAPTION= <scene name='initialview01'>1i6v</scene>, resolution 3.3&Aring;
+The line below this paragraph, containing "STRUCTURE_1i6v", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=RFP:RIFAMPICIN'>RFP</scene>, <scene name='pdbligand=UNK:UNKNOWN'>UNK</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1i6v|  PDB=1i6v  |  SCENE=  }}
-|RELATEDENTRY=[[1hqm|1HQM]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i6v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i6v OCA], [http://www.ebi.ac.uk/pdbsum/1i6v PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1i6v RCSB]</span>
-}}
 '''THERMUS AQUATICUS CORE RNA POLYMERASE-RIFAMPICIN COMPLEX'''
@@ Line 33: / Line 30: @@
 [[Category: Mustaev, A.]]
 [[Category: 3d- structure]]
-[[Category: dna-directed rna polymerase]]
+[[Category: Dna-directed rna polymerase]]
-[[Category: transcription]]
+[[Category: Transcription]]
-[[Category: transferase]]
+[[Category: Transferase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 19:38:35 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:15:24 2008''