7dzf: Difference between revisions

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<StructureSection load='7dzf' size='340' side='right'caption='[[7dzf]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
<StructureSection load='7dzf' size='340' side='right'caption='[[7dzf]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[7dzf]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DZF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DZF FirstGlance]. <br>
<table><tr><td colspan='2'>[[7dzf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DZF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DZF FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HP9:(2S)-2-azanyl-3-(7-fluoranyl-9-oxidanylidene-xanthen-2-yl)propanoic+acid'>HP9</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HP9:'>HP9</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7dze|7dze]]</div></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dzf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dzf OCA], [https://pdbe.org/7dzf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dzf RCSB], [https://www.ebi.ac.uk/pdbsum/7dzf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dzf ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dzf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dzf OCA], [https://pdbe.org/7dzf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dzf RCSB], [https://www.ebi.ac.uk/pdbsum/7dzf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dzf ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/FABPL_HUMAN FABPL_HUMAN]] Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport.  
[[https://www.uniprot.org/uniprot/FABPL_HUMAN FABPL_HUMAN]] Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
One of the primary objectives in chemistry research is to observe atomic motions during reactions in real time. Although X-ray free-electron lasers (XFELs) have facilitated the capture of reaction intermediates using time-resolved serial femtosecond crystallography (TR-SFX), only a few natural photoactive proteins have been investigated using this method, mostly due to the lack of suitable phototriggers. Here we report the genetic encoding of a xanthone amino acid (FXO), as an efficient phototrigger, into a rationally designed human liver fatty-acid binding protein mutant (termed XOM), which undergoes photo-induced C-H bond transformation with high selectivity and quantum efficiency. We solved the structures of XOM before and 10-300 ns after flash illumination, at 1.55-1.70 A resolutions, and captured the elusive excited-state intermediates responsible for precise C-H bond activation. We expect that most redox enzymes can now be investigated by TR-SFX, using our method, to reveal reaction intermediates key for their efficiency and selectivity.
 
Excited-state intermediates in a designer protein encoding a phototrigger caught by an X-ray free-electron laser.,Liu X, Liu P, Li H, Xu Z, Jia L, Xia Y, Yu M, Tang W, Zhu X, Chen C, Zhang Y, Nango E, Tanaka R, Luo F, Kato K, Nakajima Y, Kishi S, Yu H, Matsubara N, Owada S, Tono K, Iwata S, Yu LJ, Shen JR, Wang J Nat Chem. 2022 Jul 18. pii: 10.1038/s41557-022-00992-3. doi:, 10.1038/s41557-022-00992-3. PMID:35851837<ref>PMID:35851837</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7dzf" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Li, H]]
[[Category: Li H]]
[[Category: Liu, X]]
[[Category: Liu X]]
[[Category: Shen, J R]]
[[Category: Shen J-R]]
[[Category: Wang, J]]
[[Category: Wang J]]
[[Category: Yu, L J]]
[[Category: Yu L-J]]
[[Category: Fabp]]
[[Category: Recombination]]

Revision as of 19:46, 7 September 2022

Intermediate of FABP with a delay time of 10 nsIntermediate of FABP with a delay time of 10 ns

Structural highlights

7dzf is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[FABPL_HUMAN] Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport.

7dzf, resolution 1.70Å

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