1i5o: Difference between revisions

Revision as of 19:36, 2 May 2008

CRYSTAL STRUCTURE OF MUTANT R105A OF E. COLI ASPARTATE TRANSCARBAMOYLASE

OverviewOverview

Regulation of protein function, often achieved by allosteric mechanisms, is central to normal physiology and cellular processes. Although numerous models have been proposed to account for the cooperative binding of ligands to allosteric proteins and enzymes, direct structural support has been lacking. Here, we used a combination of X-ray crystallography and small angle X-ray scattering in solution to provide direct structural evidence that the binding of ligand to just one of the six active sites of Escherichia coli aspartate transcarbamoylase induces a concerted structural transition from the T to the R state.

About this StructureAbout this Structure

1I5O is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Direct structural evidence for a concerted allosteric transition in Escherichia coli aspartate transcarbamoylase., Macol CP, Tsuruta H, Stec B, Kantrowitz ER, Nat Struct Biol. 2001 May;8(5):423-6. PMID:11323717 Page seeded by OCA on Fri May 2 19:36:22 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1i5o.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1i5o |SIZE=350|CAPTION= <scene name='initialview01'>1i5o</scene>, resolution 2.8&Aring;
+The line below this paragraph, containing "STRUCTURE_1i5o", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=PAL:N-(PHOSPHONACETYL)-L-ASPARTIC+ACID'>PAL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_carbamoyltransferase Aspartate carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.2 2.1.3.2] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= PYRB, PYRI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+-->
-|DOMAIN=
+{{STRUCTURE_1i5o|  PDB=1i5o  |  SCENE=  }}
-|RELATEDENTRY=[[5at1|5at1]], [[6at1|6at1]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i5o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i5o OCA], [http://www.ebi.ac.uk/pdbsum/1i5o PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1i5o RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF MUTANT R105A OF E. COLI ASPARTATE TRANSCARBAMOYLASE'''
@@ Line 30: / Line 27: @@
 [[Category: Stec, B.]]
 [[Category: Tsuruta, H.]]
-[[Category: mutant aspartate transcarbamoylase]]
+[[Category: Mutant aspartate transcarbamoylase]]
-[[Category: pala at the regulatory site]]
+[[Category: Pala at the regulatory site]]
-[[Category: t-state]]
+[[Category: T-state]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 19:36:22 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:14:59 2008''