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==Crystal structure of ProMacrobody 21 with bound maltose==
==Crystal structure of ProMacrobody 21 with bound maltose==
<StructureSection load='7omt' size='340' side='right'caption='[[7omt]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
<StructureSection load='7omt' size='340' side='right'caption='[[7omt]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[7omt]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OMT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OMT FirstGlance]. <br>
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OMT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OMT FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene></td></tr>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7omt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7omt OCA], [https://pdbe.org/7omt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7omt RCSB], [https://www.ebi.ac.uk/pdbsum/7omt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7omt ProSAT]</span></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7omm|7omm]]</div></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7omt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7omt OCA], [https://pdbe.org/7omt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7omt RCSB], [https://www.ebi.ac.uk/pdbsum/7omt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7omt ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Lipopolysaccharides are major constituents of the extracellular leaflet in the bacterial outer membrane and form an effective physical barrier for environmental threats and for antibiotics in Gram-negative bacteria. The last step of LPS insertion via the Lpt pathway is mediated by the LptD/E protein complex. Detailed insights into the architecture of LptDE transporter complexes have been derived from X-ray crystallography. However, no structure of a laterally open LptD transporter, a transient state that occurs during LPS release, is available to date. Here, we report a cryo-EM structure of a partially opened LptDE transporter in complex with rigid chaperones derived from nanobodies, at 3.4 A resolution. In addition, a subset of particles allows to model a structure of a laterally fully opened LptDE complex. Our work offers insights into the mechanism of LPS insertion, provides a structural framework for the development of antibiotics targeting LptD and describes a highly rigid chaperone scaffold to enable structural biology of challenging protein targets.
Cryo-EM structures of a LptDE transporter in complex with Pro-macrobodies offer insight into lipopolysaccharide translocation.,Botte M, Ni D, Schenck S, Zimmermann I, Chami M, Bocquet N, Egloff P, Bucher D, Trabuco M, Cheng RKY, Brunner JD, Seeger MA, Stahlberg H, Hennig M Nat Commun. 2022 Apr 5;13(1):1826. doi: 10.1038/s41467-022-29459-2. PMID:35383177<ref>PMID:35383177</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7omt" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Bocquet, N]]
[[Category: Bocquet N]]
[[Category: Botte, M]]
[[Category: Botte M]]
[[Category: Brunner, J D]]
[[Category: Brunner JD]]
[[Category: Bucher, D]]
[[Category: Bucher D]]
[[Category: Chami, M]]
[[Category: Chami M]]
[[Category: Cheng, R K.Y]]
[[Category: Cheng RKY]]
[[Category: Egloff, P]]
[[Category: Egloff P]]
[[Category: Hennig, M]]
[[Category: Hennig M]]
[[Category: Ni, D]]
[[Category: Ni D]]
[[Category: Schenck, S]]
[[Category: Schenck S]]
[[Category: Seeger, M A]]
[[Category: Seeger MA]]
[[Category: Stahlberg, H]]
[[Category: Stahlberg H]]
[[Category: Trabuco, M]]
[[Category: Trabuco M]]
[[Category: Zimmermann, I]]
[[Category: Zimmermann I]]
[[Category: Immune system]]
[[Category: Nanobody cryo-em chaperone mbp]]

Revision as of 17:47, 31 August 2022

Crystal structure of ProMacrobody 21 with bound maltoseCrystal structure of ProMacrobody 21 with bound maltose

Structural highlights

Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT
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