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| ==Structure of the Exo-L-galactose-6-sulfatase BuS1_11 from Bacteroides uniformis== | | ==Structure of the Exo-L-galactose-6-sulfatase BuS1_11 from Bacteroides uniformis== |
| <StructureSection load='7lha' size='340' side='right'caption='[[7lha]], [[Resolution|resolution]] 1.95Å' scene=''> | | <StructureSection load='7lha' size='340' side='right'caption='[[7lha]]' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[7lha]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LHA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LHA FirstGlance]. <br> | | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LHA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LHA FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr> | | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lha FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lha OCA], [https://pdbe.org/7lha PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lha RCSB], [https://www.ebi.ac.uk/pdbsum/7lha PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lha ProSAT]</span></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lha FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lha OCA], [https://pdbe.org/7lha PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lha RCSB], [https://www.ebi.ac.uk/pdbsum/7lha PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lha ProSAT]</span></td></tr> | |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| Native porphyran is a hybrid of porphryan and agarose. As a common element of edible seaweed, this algal galactan is a frequent component of the human diet. Bacterial members of the human gut microbiota have acquired polysaccharide utilization loci (PULs) that enable the metabolism of porphyran or agarose. However, the molecular mechanisms that underlie the deconstruction and use of native porphyran remains incompletely defined. Here, we have studied two human gut bacteria, porphyranolytic Bacteroides plebeius and agarolytic Bacteroides uniformis, that target native porphyran. This reveals an exo-based cycle of porphyran depolymerization that incorporates a keystone sulfatase. In both PULs this cycle also works together with a PUL-encoded agarose depolymerizing machinery to synergistically reduce native porphyran to monosaccharides. This provides a framework for understanding the deconstruction of a hybrid algal galactan, and insight into the competitive and/or syntrophic relationship of gut microbiota members that target rare nutrients.
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| Metabolism of a hybrid algal galactan by members of the human gut microbiome.,Robb CS, Hobbs JK, Pluvinage B, Reintjes G, Klassen L, Monteith S, Giljan G, Amundsen C, Vickers C, Hettle AG, Hills R, Nitin, Xing X, Montina T, Zandberg WF, Abbott DW, Boraston AB Nat Chem Biol. 2022 Mar 14. pii: 10.1038/s41589-022-00983-y. doi:, 10.1038/s41589-022-00983-y. PMID:35289327<ref>PMID:35289327</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 7lha" style="background-color:#fffaf0;"></div>
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| ==See Also== | | ==See Also== |
| *[[Sulfatase 3D structures|Sulfatase 3D structures]] | | *[[Sulfatase 3D structures|Sulfatase 3D structures]] |
| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Boraston, A B]] | | [[Category: Boraston AB]] |
| [[Category: Robb, C S]] | | [[Category: Robb CS]] |
| [[Category: Carbohydrate]]
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| [[Category: Hydrolase]]
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| [[Category: Porphyran]]
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| [[Category: Sulfatase]]
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