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==Conserved Residues that Modulate Protein trans-Splicing of Npu DnaE Split Intein==
==Conserved Residues that Modulate Protein trans-Splicing of Npu DnaE Split Intein==
<StructureSection load='4lx3' size='340' side='right'caption='[[4lx3]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
<StructureSection load='4lx3' size='340' side='right'caption='[[4lx3]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4lx3]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LX3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LX3 FirstGlance]. <br>
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LX3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LX3 FirstGlance]. <br>
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lx3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lx3 OCA], [https://pdbe.org/4lx3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lx3 RCSB], [https://www.ebi.ac.uk/pdbsum/4lx3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lx3 ProSAT]</span></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lx3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lx3 OCA], [https://pdbe.org/4lx3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lx3 RCSB], [https://www.ebi.ac.uk/pdbsum/4lx3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lx3 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The first crystal trans-structure of a naturally occurring split intein has been determined for the Npu DnaE split intein. Guided by this structure, the residues NArg50 and CSer35, well conserved in DnaE split inteins, are identified to be critical in the trans-splicing of Npu DnaE split intein. An in vitro splicing assay demonstrates that NArg50 and CSer35 play synergistic roles in modulating its intein activity. The C-terminal asparagine CAsn36 exhibits two orientations of its side-chain and interacts with both NArg50 and CSer35 through H-bonding. These interactions likely facilitate cyclization of Asn in the course of protein splicing. The mutation of either residue reduces intein activity, and correlates with the low activity of the Ssp DnaE split intein. On the other hand, NArg50 also forms a H-bond with the highly conserved F-block aspartate CAsp17, thus influencing the N-S acyl shift during N-terminal cleavage. Sequence alignments show that residues NArg50 and CSer35 are rather conserved in those split inteins that lack a penultimate histidine residue. The conserved non-catalytic residues of split inteins modulate the efficiency of protein trans-splicing by H-bond interactions with the catalytic residues at the splice junction.
Conserved Residues that Modulate Protein trans-Splicing of Npu DnaE Split Intein.,Wu Q, Gao ZQ, Wei Y, Ma G, Zheng Y, Dong YH, Liu Y Biochem J. 2014 Apr 23. PMID:24758175<ref>PMID:24758175</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4lx3" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: DNA-directed DNA polymerase]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Dong, Y]]
[[Category: Dong Y]]
[[Category: Gao, Z]]
[[Category: Gao Z]]
[[Category: Liu, Y]]
[[Category: Liu Y]]
[[Category: Ma, G]]
[[Category: Ma G]]
[[Category: Wei, Y]]
[[Category: Wei Y]]
[[Category: Wu, Q]]
[[Category: Wu Q]]
[[Category: Zheng, Y]]
[[Category: Zheng Y]]
[[Category: Naturally occurring]]
[[Category: Trans-structure]]
[[Category: Transferase]]

Latest revision as of 13:47, 31 August 2022

Conserved Residues that Modulate Protein trans-Splicing of Npu DnaE Split InteinConserved Residues that Modulate Protein trans-Splicing of Npu DnaE Split Intein

Structural highlights

Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT
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OCA
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