|
|
| Line 1: |
Line 1: |
|
| |
|
| ==Solution structure of FimA wt== | | ==Solution structure of FimA wt== |
| <StructureSection load='2m5g' size='340' side='right'caption='[[2m5g]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | | <StructureSection load='2m5g' size='340' side='right'caption='[[2m5g]]' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[2m5g]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M5G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M5G FirstGlance]. <br> | | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M5G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M5G FirstGlance]. <br> |
| </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m5g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m5g OCA], [https://pdbe.org/2m5g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m5g RCSB], [https://www.ebi.ac.uk/pdbsum/2m5g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m5g ProSAT]</span></td></tr> | | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m5g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m5g OCA], [https://pdbe.org/2m5g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m5g RCSB], [https://www.ebi.ac.uk/pdbsum/2m5g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m5g ProSAT]</span></td></tr> |
| </table> | | </table> |
| == Function ==
| |
| [[https://www.uniprot.org/uniprot/FIMA1_ECOLI FIMA1_ECOLI]] Fimbriae (also called pili), polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell, enable bacteria to colonize the epithelium of specific host organs.
| |
| <div style="background-color:#fffaf0;">
| |
| == Publication Abstract from PubMed ==
| |
| Type 1 pili are filamentous organelles mediating the attachment of uropathogenic Escherichia coli to epithelial cells of host organisms. The helical pilus rod consists of up to 3000 copies of the main structural subunit FimA that interact via donor strand complementation, where the incomplete Ig-like fold of FimA is completed by insertion of the N-terminal extension (donor strand) of the following FimA subunit. Recently, it was shown that FimA also exists in a monomeric, assembly-incompetent form and that FimA monomers act as inhibitors of apoptosis in infected host cells. Here we present the NMR structure of monomeric wild-type FimA with its natural N-terminal donor strand complementing the Ig fold. Compared to FimA subunits in the assembled pilus, intramolecular self-complementation in the monomer stabilizes the FimA fold with significantly less interactions, and the natural FimA donor strand is inserted in the opposite orientation. In addition, we show that a motif of two glycine residues in the FimA donor strand, separated by five residues, is the prerequisite of the alternative, parallel donor strand insertion mechanism in the FimA monomer and that this motif is preserved in FimA homologs of many enteroinvasive pathogens. We conclude that FimA is a unique case of a protein with alternative, functionally relevant folding possibilities, with the FimA polymer forming the highly stable pilus rod and the FimA monomer promoting pathogen propagation by apoptosis suppression of infected epithelial target cells.
| |
|
| |
| Intramolecular Donor Strand Complementation in the E. coli Type 1 Pilus Subunit FimA Explains the Existence of FimA Monomers As Off-Pathway Products of Pilus Assembly That Inhibit Host Cell Apoptosis.,Walczak MJ, Puorger C, Glockshuber R, Wider G J Mol Biol. 2013 Oct 30. pii: S0022-2836(13)00686-4. doi:, 10.1016/j.jmb.2013.10.029. PMID:24184277<ref>PMID:24184277</ref>
| |
|
| |
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| |
| </div>
| |
| <div class="pdbe-citations 2m5g" style="background-color:#fffaf0;"></div>
| |
|
| |
|
| ==See Also== | | ==See Also== |
| *[[Pilin 3D structures|Pilin 3D structures]] | | *[[Pilin 3D structures|Pilin 3D structures]] |
| == References ==
| |
| <references/>
| |
| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Glockshuber, R]] | | [[Category: Glockshuber R]] |
| [[Category: Puorger, C]] | | [[Category: Puorger C]] |
| [[Category: Walczak, M J]] | | [[Category: Walczak MJ]] |
| [[Category: Wider, G]] | | [[Category: Wider G]] |
| [[Category: Fima]]
| |
| [[Category: Pili]]
| |
| [[Category: Pilus]]
| |
| [[Category: Structural protein]]
| |