1i1h: Difference between revisions

Revision as of 19:27, 2 May 2008

CRYSTAL STRUCTURE ANALYSIS OF PRECORRIN-8X METHYLMUTASE COMPLEX WITH HYDROGENOBYRINIC ACID

OverviewOverview

BACKGROUND: The crystal structure of precorrin-8x methyl mutase (CobH), an enzyme of the aerobic pathway to vitamin B12, provides evidence that the mechanism for methyl migration can plausibly be regarded as an allowed [1,5]-sigmatropic shift of a methyl group from C-11 to C-12 at the C ring of precorrin-8x to afford hydrogenobyrinic acid. RESULTS: The dimeric structure of CobH creates a set of shared active sites that readily discriminate between different tautomers of precorrin-8x and select a discrete tautomer for sigmatropic rearrangement. The active site contains a strictly conserved histidine residue close to the site of methyl migration in ring C of the substrate. CONCLUSION: Analysis of the structure with bound product suggests that the [1,5]-sigmatropic shift proceeds by protonation of the ring C nitrogen, leading to subsequent methyl migration.

About this StructureAbout this Structure

1I1H is a Single protein structure of sequence from Pseudomonas denitrificans. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of precorrin-8x methyl mutase., Shipman LW, Li D, Roessner CA, Scott AI, Sacchettini JC, Structure. 2001 Jul 3;9(7):587-96. PMID:11470433 Page seeded by OCA on Fri May 2 19:27:47 2008

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OCA

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 [[Image:1i1h.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1i1h |SIZE=350|CAPTION= <scene name='initialview01'>1i1h</scene>, resolution 2.60&Aring;
+The line below this paragraph, containing "STRUCTURE_1i1h", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=COJ:HYDROGENOBYRINIC+ACID'>COJ</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Precorrin-8X_methylmutase Precorrin-8X methylmutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.1.2 5.4.1.2] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= COBH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=43306 Pseudomonas denitrificans])
+-->
-|DOMAIN=
+{{STRUCTURE_1i1h|  PDB=1i1h  |  SCENE=  }}
-|RELATEDENTRY=[[1f2v|1F2V]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i1h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i1h OCA], [http://www.ebi.ac.uk/pdbsum/1i1h PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1i1h RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE ANALYSIS OF PRECORRIN-8X METHYLMUTASE COMPLEX WITH HYDROGENOBYRINIC ACID'''
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 [[Category: Scott, A I.]]
 [[Category: Shipman, L W.]]
-[[Category: precorrin]]
+[[Category: Precorrin]]
-[[Category: vitamin b12]]
+[[Category: Vitamin b12]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 19:27:47 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:13:23 2008''