1i1a: Difference between revisions

Revision as of 19:27, 2 May 2008

CRYSTAL STRUCTURE OF THE NEONATAL FC RECEPTOR COMPLEXED WITH A HETERODIMERIC FC

OverviewOverview

The neonatal Fc receptor (FcRn) transports immunoglobulin G (IgG) across epithelia, binding IgG in acidic vesicles (pH < or = 6.5) and releasing IgG in the blood at pH 7.4. Well-ordered FcRn/Fc crystals are prevented by the formation of "oligomeric ribbons" of FcRn dimers bridged by Fc homodimers, thus we crystallized a 1:1 complex between rat FcRn and a heterodimeric Fc containing only one FcRn binding site. The 2.8 A complex structure demonstrates that FcRn uses its alpha2 and beta2-microglobulin domains and carbohydrate to interact with the Fc C(gamma)2-C(gamma)3 interface. The structure reveals conformational changes in Fc and three titratable salt bridges that confer pH-dependent binding, and can be used to guide rational design of therapeutic IgGs with longer serum half-lives.

About this StructureAbout this Structure

1I1A is a Protein complex structure of sequences from Rattus norvegicus. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure at 2.8 A of an FcRn/heterodimeric Fc complex: mechanism of pH-dependent binding., Martin WL, West AP Jr, Gan L, Bjorkman PJ, Mol Cell. 2001 Apr;7(4):867-77. PMID:11336709 Page seeded by OCA on Fri May 2 19:27:22 2008

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OCA

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 [[Image:1i1a.gif|left|200px]]
- {{Structure
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i1a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i1a OCA], [http://www.ebi.ac.uk/pdbsum/1i1a PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1i1a RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF THE NEONATAL FC RECEPTOR COMPLEXED WITH A HETERODIMERIC FC'''
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 [[Category: Jr., A P.West.]]
 [[Category: Martin, W L.]]
-[[Category: ig constant domain]]
+[[Category: Ig constant domain]]
-[[Category: mhc class i fold]]
+[[Category: Mhc class i fold]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 19:27:22 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:13:14 2008''