4air: Difference between revisions
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<StructureSection load='4air' size='340' side='right'caption='[[4air]], [[Resolution|resolution]] 1.80Å' scene=''> | <StructureSection load='4air' size='340' side='right'caption='[[4air]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4air]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4air]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leima Leima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AIR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AIR FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FGA:GAMMA-D-GLUTAMIC+ACID'>FGA</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FGA:GAMMA-D-GLUTAMIC+ACID'>FGA</scene></td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Cysteine_synthase Cysteine synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.47 2.5.1.47] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4air FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4air OCA], [https://pdbe.org/4air PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4air RCSB], [https://www.ebi.ac.uk/pdbsum/4air PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4air ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Cysteine synthase]] | [[Category: Cysteine synthase]] | ||
[[Category: Large Structures]] | |||
[[Category: Leima]] | [[Category: Leima]] | ||
[[Category: Coombs, G H]] | [[Category: Coombs, G H]] | ||
Revision as of 08:35, 25 August 2022
Leishmania major cysteine synthaseLeishmania major cysteine synthase
Structural highlights
Publication Abstract from PubMedCysteine biosynthesis is a potential target for drug development against parasitic Leishmania species; these protozoa are responsible for a range of serious diseases. To improve understanding of this aspect of Leishmania biology, a crystallographic and biochemical study of L. major cysteine synthase has been undertaken, seeking to understand its structure, enzyme activity and modes of inhibition. Active enzyme was purified, assayed and crystallized in an orthorhombic form with a dimer in the asymmetric unit. Diffraction data extending to 1.8 A resolution were measured and the structure was solved by molecular replacement. A fragment of gamma-poly-D-glutamic acid, a constituent of the crystallization mixture, was bound in the enzyme active site. Although a D-glutamate tetrapeptide had insignificant inhibitory activity, the enzyme was competitively inhibited (K(i) = 4 microM) by DYVI, a peptide based on the C-terminus of the partner serine acetyltransferase with which the enzyme forms a complex. The structure surprisingly revealed that the cofactor pyridoxal phosphate had been lost during crystallization. Structure of Leishmania major cysteine synthase.,Fyfe PK, Westrop GD, Ramos T, Muller S, Coombs GH, Hunter WN Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jul 1;68(Pt 7):738-43., Epub 2012 Jun 22. PMID:22750854[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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